血栓闭塞性脉管炎患者病变血管壁中的巨细胞病毒DNA(英文)

Cytomegalovirus DNA in involved vascular wall of patients with thromboangiitis obliterans

目的 巨细胞病毒广泛存在 ,人和动物感染后可导致多种血管疾病。血栓闭塞性脉管炎 ,作为一种血管疾病 ,其病因未明 ,本研究的目的在于考察该病与巨细胞病毒感染的相关性。 方法 为了考查血栓闭塞性脉管炎病变血管壁中巨细胞病毒DNA的存在 ,以PCR方法分析了 41例该病血管壁标本和 14例正常血管壁标本 ,引物基于该病毒即刻早期基因上游增强子的核酸序列。 结果 41例病变血管标本中有 2 8例 (6 9.2 9% )发现巨细胞病毒核酸的存在 ,14例正常血管壁中有 2例 (14.2 9% )。两组间巨细胞病毒的感染率有统计学差异 (P <0 .0 5 )。血栓闭塞性脉管炎病变血管壁的部位与巨细胞病毒感染率无关 (P >0 .0 5 )。血栓闭塞性脉管炎吸烟患者与非吸烟患者病变血管壁中巨细胞病毒感染率无统计学差异 (P >0 .0 5 )。 结论 结果显示 ,在血栓闭塞性脉管炎病变血管壁中存在巨细胞病毒感染 ,它的感染可能与该病的发生和 (或 )

Objective Cytomegalovirus, being ubiquitous, may induce infection with involvement of vessel wall in animal and human. The cause of thromboangiitis obliterans, as one of the vessel wall diseases, is still unclear. The objective of this research was to explore the relationship between cytomegalovirus infection and thromboangiitis obliterans. Methods To investigate the presence of cytomegalovirus DNA in the pathological vascular wall of thromboangiitis obliterans, 41 involved vascular specimens from patients who underwent vascular surgery for this disease and 14 normal vascular specimens were analyzed by polymerase chain reaction. The primer pair was based on the nucleic acid sequence of an enhancer located in upstream of an immediate early gene of cytomegalovirus. Results With this primer pair, cytomegalovirus nucleic acid was found in 28(68 29%) of 41 pathologic vascular specimens and 2(14 29%) of 14 normal vascular specimens. The difference between the two groups had statistic significance ( P <0 05). The infection rate of cytomegalovirus was correlated neither with the sites of vessel wall of thromboangiitis obliterans, nor with the smoking status of the patients. Conclusions The results suggest that there is cytomegalovirus infection in the pathological vascular wall of thromboangiitis obliterans. Cytomegalovirus infection may be associated with pathogenesis and /or development of thromboangiitis obliterans.