摘要
根据已报道的物种GHR基因cDNA序列设计引物,利用RT-PCR技术从水貂肝脏组织总RNA中扩增出GHR基因的cDNA序列,并进行序列分析。结果表明,RT-PCR扩增出的cDNA片段为826bp,利用邻接法构建了分子系统进化树。
The gene encoding Mink growth hormone receptor（GHR） was amplified by RT- PCR from the liver total RNA. The PCR product was sequenced and analyzed subsequently. The results indicated that length of mGHR cDNA fragment was 826bp in length, the molecular phyloge- netic tree was constructed by the NJ method based on GHR gene sequences.
出处
《特产研究》
2014年第1期1-4,共4页
Special Wild Economic Animal and Plant Research
基金
公益性行业(农业)科研专项经费(200903014)
吉林市科技支撑计划(201162513)
