摘要
目的 探讨非多巴胺能组织 -羊膜细胞移植治疗帕金森病 (PD)的作用机制。方法通过 6 -羟基多巴胺 (6 -OHAD)立体定向注射破坏一侧黑质 ,制成PD大鼠模型 ,然后将鼠成活羊膜细胞悬液植入受损侧纹状体 ,与死羊膜细胞移植及生理盐水假移植对照。结果发现活羊膜细胞移植可抑制大鼠的异常旋转行为 ,酪氨酸羟化酶 (TH)免疫组化结果显示在活羊膜细胞移植靶点周围出现了TH阳性物质。结论活羊膜细胞诱导了宿主纹状体残存多巴胺 (DA)神经纤维的再生 ,提示羊膜细胞能够分泌对在体DA神经元具有营养作用的物质。
Objective To study the mechanism of amnion cell transplants in the treatment of Parkinson's disease Methods After the substantia nigra of rats was damaged unilaterally with 60 HDA, live-amnion cells were implanted into the denervated striatum Results Rats implanted with live amnion cells had significant mitigation of turning in response to apomorphine, and the implanted striatum with live-amnion cells transplant contains significantly greater TH-immunoreactive fibers than those of the killed-amnion-cells or saline groups Conclusions The results indicate that the live amnion cells can evoke the regeneration of dopaminergic fibers in the denervated striatum which may be through a trophic mechanism It suggests that the neurotrophic factor may play a role in the therapeutic mechanisms of tissue grafts on Parkinson's disease
出处
《第一军医大学学报》
CSCD
1999年第6期527-529,共3页
Journal of First Military Medical University
