血清心肌肌钙蛋白I对急性病毒性心肌炎心肌损伤诊断的价值

Experiment on the role of serum cardiac troponin I in detecting myocardial damage of acute viral myocarditis

目的 :探讨病毒性心肌炎 (VMC)小鼠血清心肌肌钙蛋白 I(c Tn I)含量与心肌组织病理改变的关系 ,为临床诊断 VMC心肌损伤提供依据。方法 :用柯萨奇 B3亲心肌病毒株 (CVB3m)制备小鼠 VMC模型 ;分别于感染病毒后第 8、15天及第 3个月 ,观测 VMC小鼠血清 c Tn I含量、心肌坏死病理组织学积分 (HSN)和心肌炎症组织学积分 (HSI) ,并探讨其相互关系。结果 :VMC小鼠心肌损伤以感染后第 8天最为显著 (P <0 .0 5 ) ,此时血清 c Tn I含量与 HSN(r =0 .6 96 ,P <0 .0 5 )及 HSI(r =0 .76 0 ,P <0 .0 5 )之间呈正相关。结论 :血清 c Tn I能确切地反映急性期 VMC小鼠心肌损伤的程度 ,是 VMC心肌损伤的可靠而敏感的检测指标。

Objective:Through investigating the relationship between serum cardiac troponin Ⅰ (cTnI) and cardiac pathological changes in viral myocarditis mice,to judge if cTnI can be served as a maker of myocardial damage in viral myocarditis (VMC) in clinic.Method:The model of viral myocarditis mice was prepared with Coxsackievirus B 3m (CVB 3m ). Then level of serum cTnI, histological scores of necrosis (HSN) and inflammation (HSI) were observed in VMC mice on day 8,15,90 after CVB 3m infection, and the relationship between cTnI and the extent of cardiac damage was described as well.Result:The HSN and HSI had the linear correlativity with the content of serum cTnI in VMC mice on 8th day after virus infection (HSN, r= 0.696 ,P< 0.05 and HSI, r= 0.760 , P< 0.05 ). Conclusion:The content of serum cTnI can closely reflect the extent of myocardial damage in acute VMC mice. Serum cTnI is a sensitive and specific marker of viral myocardial damage.