人胚胎外周组织褪黑素受体的鉴定

Identification of melatonin receptor in human embryonic peripheral tissue

目的 :探讨人胚胎外周组织褪黑素 (Mel)受体 (MR)体内分布的蛋白结合活性及信号转导途径。 方法 :应用放射配体结合法检测人胚胎外周组织 MR,并以 GTPγS观察其对 Mel特异结合的影响。 结果 :经饱和分析、动力学分析及特异性分析证明人胚胎心、肝、肾及甲状腺等 16种组织均存在 Mel特异结合位点 ,亚细胞分布研究表明这些组织中以细胞核及线粒体MR含量最高 ;GTPγS能够抑制 Mel与其受体的结合 ,使 MR的结合容量或亲和力降低。结论 :MR在人胚胎组织存在广泛 ,为解释 Mel作用的广泛性提供了实验依据。实验结果支持 MR是由抑制性

Objective: To determine protein binding characteristic and signal transmission pathway of melatonin(Mel) receptor(MR) in human embryonic peripheral organ tissues. Methods: MR was measured by radio ligand binding assay and the effect of GTPγS on melatonin specific binding was studied. Results: Mel specific binding sites were determined in 16 kinds of human embryonic tissue and this binding could be inhibited by GTPγS, supporting the theory that MR is coupled to inhibitory G proteins system. Conclusion: MR is measured in human embryo tissue, these results provide experimental data for elucidating the mechanism of the effect of Mel.