急性脑梗死患者外周血白细胞褪黑素受体昼夜节律的变化

The circadian ryhthm variation of melatonin receptor in peripheral leukocytes in patients with acute cerebral infarction

目的 :探讨急性脑梗死时褪黑素受体 (MR)结合容量的变化及 MR昼夜节律的改变。 方法 :选择正常老年人 15名[年龄 (6 8.33± 7.76岁 ) ]作为对照组 ,急性脑梗死患者 17例 [年龄 (70 .18± 11.70 )岁 ],采用放射配体结合法测定 12 :0 0 (光中期 )和 2 4:0 0 (暗中期 )外周血白细胞 MR的结合容量 ,比较两组 MR的结合容量及昼夜节律变化。 结果 :Scatchard分析和一点法分析均显示 ,急性脑梗死组光中期 MR结合容量明显低于对照组 ,分别为 (0 .14± 0 .0 5 ) fmol/ 10 6 和 (0 .2 3± 0 .0 3)fm ol/ 10 6 ,及 (0 .0 95± 0 .0 41) fm ol/ 10 6 和 (0 .16 0± 0 .0 49) fmol/ 10 6 ,两组差异非常显著 (P<0 .0 5和 P<0 .0 1) ;但两组 MR结合的亲和力无明显改变 (P>0 .0 5 )。对照组光中期的 MR结合容量明显高于暗中期 (P<0 .0 1) ,而急性脑梗死组光中期与暗中期比较无显著差异 (P>0 .0 5 )。 结论 :急性脑梗死时 MR表达下降 。

Objective: To investigate the circadian ryhthm variation of 2 125 I iodomelatonin ( 125 I Mel) binding sites in the human peripheral leukocytes in patients with acute cerebral infarction. Methods: Melatonin binding sites in the human peripheral leukocytes were studied using 125 I Mel as a radioligand (radioligand binding assays).All patients [age: (70.18± 11.70) years] were diagnosed by CT according to the standard [Chin J Nerv, 1996,29(6):379]. We also studied 15 age matched healthy old people as the control [age: (68.33±7.76) years]. Results: The circadian ryhthm variation of 125 I Mel specific binding in control remained significant ( P <0.01) with a higher value at midlight [(0.16±0.049) fmol/10 6] than at middark [(0.078± 0.035) fmol/10 6] with one point analysis. There was no significant variation in the patient group ( P >0.05). The specific binding in the peripheral leukocytes at midlight in the patients with acute cerebral infarction were lower than that of the control. Conclusion: The expression of Mel receptor decreases in the patients with acute cerebral infarction and the circadian ryhthm variation of 2 125 I Mel appears abnormal.