摘要
目的了解2012年广西壮族自治区(广西)罗城县诺如病毒流行株基因型谱分布。方法 2012年收集323例5岁以下腹泻患儿粪便标本,反转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测诺如病毒,对VP1区部分核苷酸序列进行测定并构建核苷酸系统进化树。结果检测323例病例粪便标本,诺如病毒阳性24份(7.43%);诺如病毒GⅠ基因组2份(8.33%),GⅠ.4和GⅠ.6各1份;GⅡ基因组22份(91.67%),GⅡ.6基因型1份(1/22),GⅡ.4基因型21份(21/22)。GⅡ.4中,18份(85.71%)属于2006b变异株簇,3份(14.29%)属于Sydney变异株簇。结论 2012年广西罗城县诺如病毒流行株基因型呈现多样性,以GⅡ.42006b变异株为优势流行株,首次检出GⅡ.4 Sydney变异株。
Objective To understand the genotypes of circulating Norovirus strains in Guangxi in 2012. Methods A total of 323 fecal samples collected from diarrheal children under 5 years old in 2012 were detected for Norovirus with RT -PCR, and corresponding sequences of partial VP1 region were analyzed and phylogenetic tree was constructed. Results Among the samples detected, twenty-four were Norovirus positive. Based on partial sequences of VP1 region, two strains belonged to genotype Ⅰ, twenty-two belonged to genotype Ⅱ. Three of 22 G Ⅱ strains collected in November and December belonged to GⅡ. 4 Sydney 2012 cluster ,eighteen strains belonged to the 2006b cluster and one belonged to the GⅡ. 6 cluster. Conclusion The circulating Norovirus genotypes were with diversity, and Norovirus G Ⅱ . 4 Sydney variant was first detected in Guangxi.
出处
《疾病监测》
CAS
2014年第1期28-31,共4页
Disease Surveillance
