摘要
目的观察姜黄素对猪链球菌诱导的小鼠脾细胞促炎性细胞因子的影响,探讨姜黄素对猪链球菌引起的急性中毒性休克的干预作用。方法采用乳酸脱氢酶释放法检测猪链球菌和姜黄素的细胞毒作用,酶联免疫吸附试验和流式细胞术检测相关炎症因子。结果所选剂量猪链球菌和姜黄素均未发现明显细胞毒作用。猪链球菌诱导脾细胞产生了促炎性细胞因子IL-6、TNF-α、IL-12和较高剂量的IFN-γ以及少量的IL-1β,同时也产生了抗炎因子IL-10。姜黄素对促炎因子都有抑制作用同时降低了促炎因子与抗炎因子的比例。结论姜黄素能够降低猪链球菌诱导小鼠脾细胞产生的促炎因子而发挥抗炎作用。
Objective To study the effect of curcumin on Streptococcus suis-induced inflammatory cytokines in mouse splenocytes and evaluate the role of curcumin in protecting septic shock induced by Streptococcus suis. Methods Lactate dehydrogenase （ LDH） releasing assay was conducted to determine cytotoxicity of Streptococcus suis and curcumin. Cytokine production was determined by ELISA and flow cytometry. Results Streptococcus suis and curcumin used at the dosage in this study did not cause significant cytotoxicity. Streptococcus suis induced IL-6, TNF-α, IL-12, little IL-β and higher level of IFN -γ production, whereas anti-inflammatory cytokine IL-10 was also produced. Curcumin significantly reduced the production of pro-inflammatory cytokines and the proportion of pro-inflammatory cytokines and IL-10. Conclusion Curcumin could inhibit Streptococcus suis-induced inflammatory response by reducing pro-inflammatory cytokines by splenocytes.
出处
《疾病监测》
CAS
2014年第1期58-61,共4页
Disease Surveillance
