含CD基因的腺病毒载体制备及体外抑瘤效果观察

Preparation of an adenovirus expressing cytosine deaminase and observation on its tumor killing effect in vitro

目的 :制备含胞嘧啶转氨酶 (CD)基因的重组腺病毒 (Ad- CD)并进行体外肿瘤的自杀基因治疗。方法 :构建含 CD或L ac Z基因的重组腺病毒载体 ,在 2 93细胞内重组包装后 ,体外感染小鼠结肠癌细胞株 C2 6 ,并给予不同浓度的前药氟胞嘧啶(5 - FC)进行肿瘤杀伤效果观察。 结果 :重组腺病毒载体构建成功。用制备的 Ad- CD液感染 C2 6细胞 ,并予以不同浓度 5 - FC后 ,肿瘤细胞生长受不同程度抑制 ,而对照组细胞和原位转 L ac Z基因的 C2 6细胞的生长未出现明显的变化。结论 :应用腺病毒载体转 CD基因进行体外肿瘤治疗效果明显 。

Objective: To prepare an adenovirus, Ad CD, expressing bacterial cytosine deaminase(CD),and use it for tumor suicide gene therapy. Methods: We constructed a recombined adenoviral vector carrying CD or LacZ. 293 packaging cell was transfected by the newly constructed plasmid and generated the infectious virus. Then the adenovirus was used to infect C26 cell in vitro . Results: The growth of Ad CD infected cell was obviously surpressed after administration of 5 FC. Conclusion: CD gene carried by adenoviral vector is effective for tumor cell suicide gene therapy in vitro .