东北地区大豆种质遗传多样性的SRAP标记分析

Genetic Diversity Analysis of Soybean Germplasm in Northeast Region of China by SRAP Markers

利用12对SRAP引物对东北地区20份大豆材料进行了遗传多样性分析。结果表明：12对引物组合共扩增出1527条多态性条带，各材料之间的遗传相似系数为0．2869～0．5980，平均相似系数为0．4443，显示了东北大豆种质丰富的遗传多样性。经UPGMA聚类分析，在阈值0．46处，可将供试材料分为6大类群，其中黑、吉两省大豆材料及国外种质问遗传相似度较高。研究表明，遗传改良和定向选择造成大豆品种间遗传背景日趋狭窄和混乱，SRAP分子标记技术能有效地分析大豆材料的遗传多样性和亲缘关系。

To study the genetic diversity of soybean germplasm in northeast region of China, 12 pairs of SRAP primers were used to screen the genetic diversity of 20 soybean lines, and a total of 1 527 SRAP polymorphic bands were detected. The ge- netic similarity coefficient among the tested cuhivars ranged from 0.286 9 to 0.598 0, averaging 0. 444 3. It shows genetic di- versity among soybean germplasm. The tested cultivars were divided into six groups based on the UPGMA cluster analysis. It indicates that there are highly genetic similarity among soybean germplasm in Heilongjiang,Jilin provinces and foreign region. It shows that genetic improvement and directional selection causes narrowness and confusion in the genetic backgrounds of soy- bean. However,SRAP maker can be applied to study the genetic diversity of soybean effectively.