大豆异黄酮和皂甙对D-氨基半乳糖所致肝损伤小鼠肝脏抗氧化活力的影响

Effects of Soy Isoflavones and Saponins on Hepatic Anti-oxidative Activity of Mice with Acute Liver Injury Induced by D-galactoamine

为研究大豆异黄酮和皂甙对D-氨基半乳糖(GalN)所致小鼠急性肝损伤的保护作用以及对肝脏抗氧化活力的影响,将试验小鼠随机分为正常组、模型组、大豆异黄酮组、大豆皂甙组及联苯双脂组(阳性对照组)。每日给药1次,连续7 d。试验末期,腹腔注射GalN建立小鼠急性肝损伤模型,比色法检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、白蛋白(ALB)以及肝脏过氧化脂质(LOOH)、丙二醛(MDA)、还原型谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)活性。结果表明:大豆异黄酮和皂甙降低GalN所致急性肝损伤小鼠血清ALT和AST活性,增高血清ALB水平,减少肝LOOH和MDA含量,升高肝组织GSH水平和SOD、CAT、GPx活性。大豆异黄酮和皂甙对GalN所致小鼠急性肝损伤具有保护作用,其机制可能与抗氧化作用有关。

For the purpose of studying the protective effect of soy isoflavones and saponins on acute liver injury induced by D- galactoamine（GalN）in mice,the experimental mice were randomly assigned to the normal control,model control, soy isofla- vone, saponin and bifendate（ positive control） groups. Animals were treated once daily for 7 days. GaIN were given intraperito- neally to the mice of groups, and then the alanine aminotransferase （ALT）, aspartate aminotransferase（ AST）, albumin（ ALB ）, lipid hydroperoxide （ LOOH ）, malondialdehyde （ MDA ）, reduced glutathione （ GSH ）, superoxide dismatase （ SOD ）, catalase （CAT） and glutathione peroxidase（GPx） were detected by the eolorimetric method. The administration with soy isoflavones and saponins reduced the serum ALT and AST activities, increased the serum ALB level, decreased the hepatic LOOH and MDA contents,and increased the SOD, CAT, GPx and GSH levels of liver in mice with acute liver injury. It is suggested that soy isoflavones and saponins have protective effects on acute liver injury induced by GaIN in mice, probably via anti-oxidative ac-tivity.