卡特丽亚兰的组织培养与快速繁殖的研究

STUDY ON TISSUE CULTURE AND RAPID PROPAGATION OF CATTLEYA IN VILRO

作者以卡特丽亚兰开花植株茎尖和试管苗茎段或叶切段为外植体 ,成功地诱导形成原球茎并获得再生植株 ,筛选出了原球茎球诱导培养基 :RE + 6mg/LBA (5mg/LBA + 0 .5～ 1mg/LNAA或 1BA) + 0 .5 %～ 1%蔗糖 ;原球茎增殖培养基 :MS(或KC) + 5mg/LBA + 0 .1(0 .5 )mg/LNAA(或 0 .5mg/L 1BA) + 0 .2 %蛋白胨和分化及育苗培养基 :KC + 0 .3mg/L(0 .5 )NAA + 10 %香蕉匀汁 + 0 .1%活性炭 .BA在原球茎的产生、增殖分化中起着主导作用 .

The clonal propagation of Cattleya was developed.PLB is obtained through culture of leaf segments and shoot tips and shoot stem of Cattl eya on KC solid medium with BA 6mg/L, NAA 0.5%～lmg/L and sucrose 0.5%～1%. proliferation of Protocorms is on MS or KC medium with BA 5mg/L and NAA 0.l～0.5mg/L or IBA 0.5mg/L and Peptone 0.2%. The medium as KC or MS with NAA 0.3 or 0.5mg/L and 10% banana extracts and 0.l% actived charcoal is better for plantlet growth. BA played a central role in the protocorm formation and differentiation.