甲烷氧化菌对煤吸附甲烷的氧化特性

Oxidation Potential of Methanotroph to the Methane being Absorbed on Coal

在煤样中加入甲烷氧化菌AEM1235菌悬液,充入CH4或CH4与O2的混合气体进行等温吸附实验以测定甲烷氧化菌对CH4的氧化能力.在两种充气条件下的吸附与脱吸附实验结果均显示出气体中CH4量显著减少和CO2增加的变化.在充气5.0 MPa、添加菌液量为18.75%条件下,充CH4气体、CH4与O2的混合气体时的CH4减少量分别可达其饱和吸附量的121.18%和244.39%,表明在高压、缺氧或充氧的条件下甲烷氧化菌对煤样中吸附的CH4均具有吸收和氧化的作用,这种作用随菌量增加或压力升高而加强,并且有O2存在可以促进甲烷氧化细菌对CH4的吸收和利用.该结果表明在地下煤层的高压和缺氧的条件下甲烷氧化细菌仍然具有较强的吸收和氧化煤层中结合态CH4的能力.

In the coal sample added Methanotrophs AEM1235 suspension, filled the CH4 or CH4 and O2 mixed gas to conduct the isothermal adsorption experiments in order to determine Methanotrophs＂ oxidation capacity on CH4. Under the two kinds of gas condition the adsorption and desorption experimental results shew that a significant reduction in the amount of CH4 and CO2 increase in the mixed gas. When the gas pressure was 5.0 MPa and the amount of the bacteria suspension add in was 18.75~, the reduction of CH4 in CH4 inflated and the mixed gas cou!d reach 121.18% and 244.39% of their saturated adsorp- tion capacity, showing that both in high pressure, anaerobic and aerobic conditions Methanotrophs in the coal sample had ad- sorption and oxidation for CH4. And these effects trended to become stronger with the bacteria amount and the gas pressure in- creasing, while O2 promoted these effects as well. Result above prompt us even in high pressure and anaerobic condition underground can Methanotroph keep its capacity for assimilating and oxidizing CH4.