香菇废弃菌棒厌氧发酵产气规律正交试验

Orthogonal Test and Regression Analysis for Biogas Production of Shii-take Cultivation Waste

为探索高碳原料厌氧发酵产气特性，在（35±1）℃条件下，采用批式厌氧消化工艺对香菇废弃菌棒进行厌氧发酵试验。采用L9（3 3）正交试验设计，研究预处理天数、接种物质量分数和料液质量分数对香菇废弃菌棒厌氧发酵产沼气的影响，得出高碳物料厌氧发酵最佳工艺组合。结果表明：高碳物料并非厌氧发酵产沼气的最佳原料，但经适当预处理后其产气性能有较大提高，TS产气率达0．16L／g，参比对照组增长128．57％。各因素对香菇废弃菌棒厌氧发酵产沼气影响的主次顺序为：预处理天数（极显著）、料液质量分数（极显著）、接种物质量分数（不显著）。同时应用多元回归理论建立多元回归模型，有助于预测香菇废弃菌棒不同发酵条件组合下的产气量。

Shii-take cultivation waste （SCW） was used as rich carbon material for anaerobic digestion under the mesospherie condition as （35 ±1 ） ℃. Orthogonal test and regression analysis L9 （33 ） were employed to determine the effects of the three factors including pretreatment period, inoculums concentration and total solids （TS） on biogas production. The optimal combination of the three factors has been achieved. The high-carbon content material was proved to be not an ideal feedstock for biogas production. However, after a certain period of pretreatment, the high carbon feedstock biogas production was improved a lot. The biogas production reached to 0. 16 L/g（TS） , increased by 128.57% compared with the control sample. The sequence of factors effecting SCW anaerobic fermentation is pretreatment period （evident）, TS （evident）, and inoeulums concentration （less evident）. A multiple regression equation was established and can be used for biogas production prediction under different conditions. Key words： Shii-take regression cultivation waste Biogas Anaerobic digestion Orthogona[ test Multiple analysis