建兰离体培养形态发生过程中蛋白质及过氧化物同工酶的研究

Study on protein and peroxidase of morphogenesis of Cymbidium ensifolium in Vitro

比较了建兰 (Cymbidiumensifolium)根状茎分化前后可溶性蛋白与过氧化物同工酶的变化 .发现可溶性蛋白质 2 3ku、 57ku、 65ku、 93ku含量稳定 ;2 1ku ,51ku ,73ku和 81ku只在根状茎时期出现 .2 8ku、 30ku在分化中出现 .过氧化物同工酶C1、C2 、C9、C10 在分化前后均有表达 ;而C3、C4 、C5、C6、C7。

The changes of water soluble proteins and peroxidases before and after differentiation of Cymbidium ensifolium s protocorms have been compared.The content of 23?ku,57?ku,65?ku and 93?ku soluble proteins are stable.21?ku,51?ku,73?ku and 81?ku proteins emerged only in the stage of rhizomoph.28?ku and 30?ku proteins appeared in the process of differentiation.The peroxidases C 1,C 2,C 9 and C 10 expressed before and after differentiation.But C 3,C 4,C 5,C 6,C 7 and C 8 appeared in the company with differentiation.