檵木中银椴苷的提取及测定

Extraction and determination of tiliroside from Loropetalum chinense

目的研究檵木中银椴苷的提取分离及对银椴苷进行质量控制。方法采用乙醇回流法提取药材，然后经过大孔树脂、减压硅胶柱色谱、洗脱，得到银椴苷单体，采用CosmosilC18柱（250mm×4．6mm，μum），流动相为甲醇．0．1％磷酸水溶液（55：45），检测波长为320nm，体积流量1．0mL／min。结果银椴苷能够很好地分离出来，质量分数为98％，银椴苷在0．041-0．513μg呈良好的线性关系（r=0．9997）；平均回收率为100．05％，RSD为1．50％。结论运用此方法能将银椴苷较好地提取分离，同时测定方法不仅操作简单、准确，且重复性好，可用于檵木中银椴苷的测定。

Objective To research the separation, extraction, and quality control oftiliroside from Loropetalum chinense. Methods The herbs were extracted using ethanol reflux extraction, and then through macroporous resin, depressure silica gel column chromatography, and elution, tiliroside monomer was obtained. Content was determined by the C18 column, mixture of methanol-0.1% phosphoric acid （55 ： 45） as mobile phase at 1.0 mL/min, and UV detection wavelength at 320 nm were used for the determination. Results Tiliroside can be a good separation of the monomer, with the content of 98%; Tiliroside was better in linear relationship in the range of 0.041 --0.513 μg （r = 0.999 7）. The recovery rate was 100.05%, RSD = 1.50%. Conclusion Using this method, tiliroside can be better extracted and separated, Meanwhile the determination method is very stable and accurate, and has good repeatability, which can be used for the quality control oftiliroside from L. chinense.