基于UPLC/Q-TOFMS的大鼠血浆磷脂成分的快速分析

Rapid Identification of Phospholipids in Rat Plasma by UPLC/Q-TOF MS

采用超高效液相色谱-四极杆飞行时间高分辨质谱(UPLC/Q-TOF MS)联用技术,对大鼠血浆磷脂提取液进行分析,建立了一种快速、简便、可靠的血浆磷脂鉴别方法。以经典的氯仿甲醇法为磷脂的提取方法,以10 mmol/L甲酸铵水溶液(A)-含10 mmol/L甲酸铵的乙腈-异丙醇(1∶1)混合溶液(B)为流动相,采用ESI源,分别在正、负离子模式下进行数据采集。根据实验结果,结合二级质谱裂解数据、元素组成、数据库匹配,鉴定出81个成分,其中包括7个PE、7个PI和1个SM,其余均为PC。所建立的液相色谱-质谱联用分析方法,可为大鼠血浆中磷脂成分的鉴别提供一种快速、简便、可靠的分析手段,以及为血浆磷脂代谢组学的研究奠定基础。

An ultra performance liquid chromatography tandem quadrupole time-of-flight mass spec- trometric method（ UPLC/Q -TOF MS） was developed for rapid separation and identification of phos- pholipids in rat plasma. The classical Bligh - Dyer was used as extraction method. The target com- pounds were separated on a BEH C18 column by gradient elution using 10 mmol/L ammonium forma- ter（A） - 10 mmol/L ammonium formate acetonitrile/isopropanol（ 1 ： 1 ） （B） as mobile phase, and detected by MS under ESI ~ and ESI- mode. 81 compounds were identified by UPLC/Q - TOF MS based on MS/MS fragment ions information, elemental composition in Masslynx 4. 1 and the Lipid Maps database, among which 7 PE, 7 PI and 1 SM were obtained, and the rest were all PC. The method was rapid, simple and credible for the identification of phospholipids in rat plasma, and could provide a foundation for the research of phospholipids metabonomics.