高效液相色谱法测定淀粉及相关食品中马来酸(酐)与富马酸的含量

Determination of Maleic Acid(Maleic Anhydride) and Fumaric Acid in Starch and Related Foods by High Performance Liquid Chromatography

建立了高效液相色谱法测定淀粉及相关食品中马来酸（酐）和富马酸的分析方法.试样采用1％氨水-甲醇（体积比1：1）溶液提取,以0.03 mol/L磷酸二氢铵（pH 2.45）溶液为流动相,采用Atlantis（R）T3 C18色谱柱进行分离,二极管阵列检测器检测.结果表明,在优化色谱条件下,两种目标物的分离良好,回收率为82.5％ ～ 103.5％,相对标准偏差小于4.3％,两者的定量下限均为2.0 mg/kg.该方法前处理简便快捷、灵敏度高、回收率和重现性良好,适用于淀粉及相关食品中马来酸（酐）及富马酸的测定.

An analytical method was developed for the determination of contents of maleic acid （ ma- leic anhydride） and fumaric acid in starch and related foods by high performance liquid chromatogra- phy （HPLC）. The samples were extracted with 1% ammonia water - methanol solution （ 1 ： 1 ）. The target compounds were separated on an Atlantis T3 C18 column using 0.03 mol/L ammonium di- hydrogen phosphate solution as mobile phase, and detected with diode - array detector. Under the optimized conditions. Two compounds were separated completely. The average spiked recoveries were in the range of 82.5% - 103.5% with RSDs less than 4. 3%. The limits of quantitation（LO- Qs） for maleic acid and fumaric acid were both 2.0 mg/kg. With the advantages of convenience, sensitivity, good recoveries and repeatability, the method was suitable for the detection of maleic acid（ maleic anhydride） and fumaric acid in starch and related foods.