摘要
建立了气相色谱-三重四极杆串联质谱(GC-MS/MS)分析烟草中8种主要生物碱的方法.样品经10% NaOH溶液浸提,二氯甲烷超声萃取后,采用GC-MS/MS多反应监测(MRM)模式测定,内标法定量.8种生物碱的回收率为93.2% ~ 99.0%,相对标准偏差为2.8%~6.7%.该方法简单快速、灵敏准确,适用于烟草样品中生物碱的快速测定.
A method was established for the determination of alkaloids in tobacco by gas chromatogra- phy- triple quardrupole mass spectrometry (GC -MS/MS). The samples were extracted by 10% NaOH and CH2C12, then determined by GC -MS/MS in multi-reaction monitoring(MRM) mode, and internal standard method was applied to quantify alkaloids. The average recoveries of alkaloids in tobacco samples were in the range of 93.2% - 99.0% with relative standard deviations of 2. 8% - 6. 7%. The method was simple, rapid and sensitive, and was suitable for the analysis of alkaloids in tobacco.
出处
《分析测试学报》
CAS
CSCD
北大核心
2014年第3期334-338,共5页
Journal of Instrumental Analysis
基金
国家烟草专卖局资金资助项目(110201202005)
云南省烟草公司科技计划项目(2013YN04)
关键词
气相色谱-三重四极杆串联质谱
多反应监测
烟草
生物碱
gas chromatography- triple quardrupole mass spectrometry
multi-reaction monitoring(MRM)
tobacco
alkaliods