气相色谱-三重四极杆串联质谱法同时测定烟草中8种生物碱

Simultaneous Determination of 8 Alkaloids in Tobacco by Gas Chromatography-Triple Quardrupole Mass Spectrometry

建立了气相色谱-三重四极杆串联质谱（GC-MS/MS）分析烟草中8种主要生物碱的方法.样品经10％ NaOH溶液浸提,二氯甲烷超声萃取后,采用GC-MS/MS多反应监测（MRM）模式测定,内标法定量.8种生物碱的回收率为93.2％ ～ 99.0％,相对标准偏差为2.8％～6.7％.该方法简单快速、灵敏准确,适用于烟草样品中生物碱的快速测定.

A method was established for the determination of alkaloids in tobacco by gas chromatogra- phy- triple quardrupole mass spectrometry （GC -MS/MS）. The samples were extracted by 10% NaOH and CH2C12, then determined by GC -MS/MS in multi-reaction monitoring（MRM） mode, and internal standard method was applied to quantify alkaloids. The average recoveries of alkaloids in tobacco samples were in the range of 93.2% - 99.0% with relative standard deviations of 2. 8% - 6. 7%. The method was simple, rapid and sensitive, and was suitable for the analysis of alkaloids in tobacco.