摘要
目的 建立测定小儿解表止咳口服液中盐酸麻黄碱含量的高效液相色谱法.方法 采用C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-含0.1%三乙胺的0.1%磷酸溶液(3:97)为流动相,流速为1.0 mL/min,检测波长为205 nm,柱温为30℃.结果 盐酸麻黄碱质量浓度在0.01~0.32 g/L范围内与峰面积呈良好线性关系,精密度RSD为0.13%,平均加样回收率为99.95%,RSD为2.22%(n=9).结论 该方法简便、快捷、重现性好,可作为小儿解表止咳口服液的质量控制方法.
Objective To establish a HPLC method for the content determination of ephedrine hydrochloride in Child Jiebiaozhike Oral Liquid. Methods The HPLC method was adopted on the C18 chromatographic column(250 mm ×4.6 mm,5 μm) with acetonitrile- 0. 1% phosphoric acid solution(containing 0. 1% triethylamine)(3:97) as the mobile phase. The flow rate was 1.0 mL/min and the column temperature was 30 ℃. The wavelength was 205 nm. Results The sample size of ephedrine hydrochloride in the range of 0.01-0.32 μg shoed the good linear relationship with the peak area, RSD of precision was 0. 13%. The average recovery rate was 99.95%, RSD 2. 22% (n = 9). Conclusion This method is simple and rapid with good reproducibility,and can be used as one of the quality control standards for Child Jiebiaozhike Oral Liquid.
出处
《中国药业》
CAS
2014年第6期37-38,共2页
China Pharmaceuticals
基金
四川省科技支撑项目(小儿解表止咳口服液品质提升及其产业化)
项目编号:2011GZX0103