60例医院获得性肺炎患者铜绿假单胞菌生物被膜形成能力的分析

Analysis of pseudomonas aeruginosa biofilm formation ability of 60 patients with hospital acquired pneumonia

目的通过研究医院获得性肺炎患者铜绿假单胞菌生物被膜形成过程,初步讨论铜绿假单胞菌生物被膜可能的干预治疗手段。方法以PA临床株为研究对象,收集第1天和第14天HAP患者下呼吸道痰标本并分离细菌,检测临床菌株细菌运动(泳动能力,颤搐运动和丛集运动)和BF形成能力的关联。结果本研究共收集病例60例,共获得PA临床株120株。共有115株菌株具有生物被膜形成能力(占95.8%),其中有96株菌株的生物被膜形成能力较弱(占80%)。生物被膜形成量与细菌泳动能力,颤搐运动及丛集运动均呈正相关(P<0.01),比较第1天与第14天收集细菌运动及生物被膜形成量均无明显差异(P>0.05)。结论我们收集的PA菌株中绝大多数具有BF形成能力,细菌运动能力越强,形成的BF量越多。本研究收集的病例临床治疗对PA生物被膜形成能力无明显影响。

Objective To study the formation process of pseudomonas aeruginosa biofilm in patients with hospital acquired pneumonia. Methods The clinical strains of PA were taken as the research object, which were collected from the first and fourteenth days in HAP patients with respiratory tract and bacteria isolated from sputum specimens. Bacteria movement was detected and BF formation ability of the association was evaluated. Results There were 120 strains isolated from PA and 115 strains with the ability of biofilm formation （95.8%）, including 96 strains of biofilm forming ability （80%）. Biofilm formation and bacterial swimming ability, twitching and swarming were positively correlated （P〈0.01）. Bacterial motility and biofilm formation showed no significant difference （P〉0.05）. Conclusion Most PA strains have BF forming ability. The stronger athletic ability is, the more amount BF formation has. The ttreatment of PA has no significant effect on biofilm formation.