面向免疫凝集微流控检测的电控混沌混合方法

Electric field control chaotic mixing method for immunoagglutination microfluidic detection system

针对当前免疫凝集微流控检测系统往往具有混合不可控、混合室结构复杂以及芯片复用率低等缺点,提出一种面向免疫凝集微流控检测的电控混沌混合方法。该方法在微流控芯片混合室壁面布置电极并施加混沌电场来驱动流体混合,通过分析选用Lorenz混沌系统作为混合控制电场,并制作了相应的混沌电场控制器。以类风湿因子在微流控检测系统中的免疫凝集检测为例,依据免疫凝集过程中2种样品液体不同的光学特性,采用吸光度检测法对此混沌混合方法实现的混合效果进行了检测验证。结果表明,该方法使得系统微混合室与受控流体系统体现出较高的控制性能,其相关系数为0.73,且在保证免疫凝集检测精度的条件下检测时间缩短48%,而芯片复用率也提高约7倍。

Aiming at the problem that most immunoagglutination microfluidic detection systems have the shortcomings of uncontrollable micromixing,complicated micromixing chamber structure and low chip reuse rate,an electric field control chaotic mixing method for immunoagglutination microfluidic detection system is proposed.The method exerts chaotic electric fields on the electrodes arranged in microfluidic chip micromixing chamber wall to drive the fluid mixing.Through analysis,the method selects the Lorenz chaotic system as the mixing control electric field.The corresponding chaotic electric field controller was made.The immunoagglutination detection of the rheumatoid factor in the microfluidic detection system was taken as an example,the mixing effect of the chaotic mixing method was verified with the absorbance detection method based on the fact that two kinds of sample liquids have different optical properties in the immune agglutination process.Experiment results indicate that the correlation coefficient is 0.73,which reflects the high control performance of the chaotic micromixing chamber to the controlled fluid system.Under the condition of guaranteeing the immune agglutination detection accuracy,the detection time is shortened by 48％,and the chip reuse rate is also increased by about 7 times.