姜黄素对重离子辐射损伤小鼠睾丸组织的防护作用研究

Study on Protective Effect of Curcumin against the Irradiation Damage of Testis in Mice Induced by ^(12)C^(6+) Ion Beams

探讨姜黄素(Curcumin,简称Cur)对重离子辐射损伤小鼠睾丸组织的防护作用。小鼠灌胃不同剂量的Cur后给予4 Gy剂量^(12)C^(6+)离子束全身单次照射。24 h后对小鼠睾丸组织形态学变化进行观察,并测定丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和过氧化氢酶(CAT)活性。结果显示:中、高浓度Cur预处理组对小鼠睾丸组织的形态具有较好的保护作用;低、中浓度Cur预处理组MDA含量显著降低(P<0.05);与单纯照射组相比,低浓度Cur预处理组SOD活性水平和中浓度Cur预处理组CA了活性水平显著提高(P<0.05)。结果表明:Cur对重离子全身辐射小鼠的抗氧化系统有一定的激活效应,对辐射损伤有一定的防护作用,其机制可能与Cur清除自由基,保护脂质和蛋白质有关。

The protective effect of Curcumin （Cur） against the irradiation damage of testis in mice induced by 12C6＋ ion beams was studied. Male Kung-Ming mice were exposed to whole body uniformly irradiated with 12C6＋ ion beams at 4 Gy after pretreated with Cur of different concentration. Then histological alterations were observed. Moreover, malonaldehyde （MDA） level, superoxide dismutase （SOD） activity and catalase （CAT） activity were also measured at 24 h after irradiation. The result showed that middle and high dose Cur pretreated have a good protective effect. Compared with irradiated group, MDA level decreased dramatically in the group of pretreatment with low and middle dose Cur （P 〈 0.05）. On the contrary, SOD activity in the pretreated group with low dose Cur significantly increased （P 〈 0.05）. Similarly, CAT activity in the pretreated group with middle dose Cur significantly increased （P 〈 0.05）. Data suggested that Cur could activate antioxidant system of testis in mice induced by12C6＋ ion beams, it have some protective effect to radiation damage. The mechanism may involve in its scavenging reactive oxidant species and then protecting lipid and protein capability.