900MHz手机射频辐射对体外细胞DNA损伤及EB病毒早期抗原的激活作用

DNA Damage in Vitro and Activation of Epstein-Barr Virus Early Antigen Induced by 900 MHz Mobile Phone Radiofrequency Radiation

[目的]研究900MHz、最大功率密度为10．644laW／cm2的手机射频辐射对体外细胞DNA损伤和病毒癌基因EB病毒早期抗原（EBV．EA）的激活作用。[方法]（1）将NIH／3T3细胞分别暴露在手机辐射下0、1、2、4、6、8、12h，并分别设定相应的正常对照组，用彗星电泳检测细胞DNA受损程度。（2）将Raji细胞分为A辐照（-）TPA（-）、B辐照（-）TPA（＋）、C辐照（＋）TPA（-）、D辐照（＋）TPA（＋）4组，接受手机辐照，强度为4h／d，实验持续4周；每周结束时，B和D两组细胞分别使用促癌剂12-0-十四烷酰佛波醇．13＋乙酸酯（TPA）1ng／mL处理48h。用免疫细胞化学检测各组细胞中EBV—EA的阳性表达情况。[结果]当NIH／3T3细胞辐照2h后，与对照组相比细胞Olive尾距有明显差异（P〈0．05），但在随后的2-12h内的各个检测时间点上，Olive尾距并没有随着时间的增加而增加（P〉0．05）；Raji细胞在实验4周后，辐照C、D组细胞的EBV—EA阳性表达率较A组明显增加（P〈0．05），并且在加入促癌剂TPA的D组，阳性表达率更加明显（P〈0．01）。[结论]900MHz手机射频辐射会对细胞DNA产生-定的损伤，并且能够激活EBV．EA的表达。

[ Objective ] To probe the DNA damage and activation of Epstein-Barr virus early antigen （EBV-EA） induced by 900MHz mobile phone radiofrequency radiation at an maximum power density of 10.644 μW/cm2. [ Methods ] NIH/3T3 cells were exposed to mobile phone radiation for 0, 1, 2, 4, 6, 8, 12 h, respectively and followed by DNA damage detection via comet assay; corresponding control groups were also set. Raji cells were divided into four groups： A, radiation（-）TPA（12-0-tetradecanoylphorbol- 13-acetate）（-）; B, radiation（-）TPA（＋）; C, radiation（＋）TPA（-）; D, radiation（＋）TPA（＋）. Group C and D were exposed to mobile phone radiation, four hours per day, for four weeks. At the end of each week, group B and D were treated with 1 ng/mL TPA for forty-eight hours. Immunocytochemistry method was used to detect the activation of EBV-EA. [ Results ] The Olive tail moment of NIH/3T3 cells were significantly increased after exposed to the designed mobile phone radiation over two hours （P 〈 0.05）. However, there were no significant increases in the other time points （P 〉 0.05）. Moreover, after being exposed for four weeks, the positive rates of EBV-EA in group C and D were higher than that in group A （P〈0.05）, and especially in the TPA treated group D showing the highest positive rate （P〈 0.01）. [ Conclusion ] Mobile phone radiofrequency radiation could cause damage to cellular DNA with the designed intensity and exposure protocol, and may activate the expression of EBV-EA.