黄芪多糖对血管性痴呆小鼠学习记忆能力的影响

Effects of Astragalus Polysaccharides on learning and memory in vascular dementia mice

目的观察黄芪多糖(APS)对血管性痴呆模型小鼠学习记忆能力的影响,并探讨其对脑组织胆碱酯酶(AchE)含量和乙酰胆碱(Ach)含量的影响。方法将40只昆明小鼠随机分成4组,每组10只,即对照组(0.9%NaCl 20.0 mL/kg)、模型组(0.9%NaCl 20.0 mL/kg)、阳性药物组(多奈哌齐0.2 mg/kg)和APS400 mg/kg组。灌胃3周后进行Morris水迷宫实验,测量记录小鼠每次的上台潜伏期、总路程、穿越站台次数和站台象限停留时间,并检测小鼠脑组织中的AchE活性和Ach含量。结果 APS组和阳性药物组小鼠的上台潜伏期和总路程明显缩短,穿越站台次数和站台象限停留时间显著增加(均P<0.05);APS组和阳性药物组小鼠脑组织中AchE活性增强、Ach含量显著降低(均P<0.05)。结论 APS能够改善血管性痴呆小鼠的学习记忆能力,增强脑组织中AchE活性,降低Ach含量,对血管性痴呆小鼠具有一定的治疗作用。

Objective To investigate the effects of Astragalus Polysaccharides on learning,memory,AchE and Ach in vascular dementia mice. Methods Forty mice were randomly divided into four groups, Astragalus Polysaccharides group （400 mg/kg） ,sham group （0.9% Nacl 20.0 mL/kg） ,donepezil group（0.2 mg/kg） and control group （0.9% Nacl 20.0 mL/kg） , ten in every group. The ascend platform latent period, all path, crossing platform number of times and platform quadrant residence time were measured after intragastric administration for three weeks. Then,AchE ac- tivity and Ach content in brain tissue were determined. Results Compared with sham group,Astragalus Polysaccha- rides group and donepezil group can shorten ascend platform latent period and all path, increase crossing platform number of times and plattorm quadrant residence time （P 〈 0.05）. Moreover, Astragalus Polysaccharides group and donepezil group can increase AchE and degrade Ach level （P 〈0.05）. Conclusion It is indicated that Astragalus polysaccharides provides good intervention on learning and memory in vascular dementia mice via enhancing the activi- ty of AchE and decreasing the content of Ach in mice brain tissue.