温度和pH对公子小丑鱼幼鱼消化酶活性的影响

Effects of temperature and pH on activity of digestive enzymes in Amphiprion ocellaris juvenile

研究了温度和pH对人工养殖下的公子小丑鱼(Amphiprion ocellaris)幼鱼的胃、肝脏、肠道中蛋白酶、淀粉酶、脂肪酶活性的影响,并对各部位消化酶活性进行比较。结果表明,在胃、肝脏、肠道中均可以检测出蛋白酶、淀粉酶、脂肪酶的活性,其中蛋白酶活性顺序为胃>肠道>肝脏,淀粉酶活性顺序为肠道>肝脏>胃,脂肪酶活性顺序为肠道>肝脏>胃。胃、肝脏、前肠、中肠、后肠蛋白酶的最适pH值分别为2.2、7.5、7.0、7.0和8.0;淀粉酶肝脏的最适pH值为6.0,其余部位均为7.0;脂肪酶肝脏的最适pH值为7.0,其余部位均为7.5。胃、肝脏、肠道的蛋白酶最适温度均为40℃;胃淀粉酶最适温度为40℃,肝淀粉酶最适温度为35℃,肠道淀粉酶最适温度为30℃;胃、肝脏、肠道的脂肪酶的最适温度均为40℃。

Effects of temperature and pH on activity of 3 kinds of digestive enzymes （protease, amylase and lipase） in stomach, liver and intestine of Amphiprion ocellaris juvenile were studied and compared under artificial culture. The results showed that activity of protease, amylase and lipase all could be detected in stomach, liver and intestine, the order of protease activity was stomach 〉 intestinal 〉 liver, the order of amylase activity was intestinal 〉 liver 〉 stomach, the order of lipase activity was intestinal 〉 liver 〉 stomach. The optimum pH values for protease, amylase and lipase were 2.2, 7.0 and 7.5 in stomaeh, 7.5, 6.0 and 7.0 in liver, 7.0, 7.0 and 7.5 in foregut, 7.0, 7.0 and 7.5 in midgut, 8.0, 7.0 and 7.5 in hindgut. The optimum temperature for protease in three organs was all 40℃, for amylase in stomach was 40℃, in liver was 35℃ and that in intestine （foregut, midgut, hindgut） was all 30℃. The optimum temperature for lipase in three digestive organs was all 40℃.