黄鳝性逆转相关基因的表达及定位分析

Analysis on expression and localization of gene related to sex reversal of Monopterus albus

为研究F25基因与黄鳝性逆转关系,首先利用荧光定量PCR技术对该基因在各期性腺组织中的表达量进行分析,然后利用原位杂交技术合成cRNA探针对F25基因进行杂交定位分析。结果显示,F25基因在II、III期卵巢中仅有微量表达,而在IV卵巢中表达开始迅速增加,同时随着卵巢发育成熟、排卵、退化表达量也呈现不同程度的升高,在精巢中该基因的表达量达到最大值;经定位分析发现,该基因在早期卵巢中基本不表达,在IV、V期卵巢中主要在体细胞中表达,而在精巢雄性生殖细胞中基本不存在F25基因的表达,由此推测F25基因可能在性逆转过程中起到一定的作用,且主要作用于性腺组织中的体细胞。

In order to research the relationship between gene F25 and Monopterus albus sex reversal, firstly, we used quantitative PCR technology to analyze the gene expression in different gonadal tissues, then used situ hybridization technique to synthesize cRNA probes and locate the F25 gene differential expression at different periods. The results showed that the gene expressed little in ovary at stage II and III, and began to increase rapidly in ovm3~ at stage IV, meanwhile, its expression also showed different degrees with the ovarian maturation, ovulation, degradation, its expression reached maximum in the testis. By positioning analysis, we found that the gene was not essential in the early ovary and mainly expressed in somatic cells in ovary at stage of IV and V, whereas there was no F25 gene expression in germ cells of the testes, which indicated that F25 gene may play a role in the process of sex reversal and affect on the somatic gonad tissues.