促红细胞生成素和血管紧张素受体拮抗剂对脑缺血再灌注后梗死体积和脑组织水肿的影响

Effects of erythropoietin and angiotensin receptor blocker on histological changes of cerebral infarction size and brain tissue water content

目的研究促红细胞生成素(EPO)和血管紧张素受体拮抗剂(ARB)对脑缺血后的脑保护作用。方法采用健康的SD大鼠,线栓法阻断一侧大脑中动脉血流2 h,再灌注24 h,建立局灶性脑缺血再灌注损伤模型(MCAO),治疗组分别予缺血再灌注前后经腹腔注射EPO 3 000 IU/(kg·d),或缬沙坦40 mg/(kg·d),评价大鼠神经功能,测定其脑梗死体积占全脑体积的百分比、脑组织的含水量。结果与缺血再灌注组相比,EPD处理组可改善神经功能缺失,减少脑梗死体积,降低梗死组织含水量,且经EPD预处理,改善更明显,差异有统计学意义(P<0.05);与缺血再灌注组相比,ARB处理组可减少脑梗死体积,降低梗死组织含水量,差异有统计学意义(P<0.05)。结论 EPD和ARB可减少脑梗死体积,降低梗死组织含水量,EPD可明显改善神经功能缺失,经EPD和ARB预处理改善更为明显,具有良好的脑保护作用。

Objective To explore the protective effects of erythropoietin （EPO） and angiotensin receptor blocker （ARB） after focal cerebral ischemia-reperfusion. Methods The model of focal cerebral ischemia was made by occluding middle cerebral artery （MCAO） for 2 h and reperfusing for 24 h in well SD rats. The rats received an ip injection of either EPO 3 000 IU/（kg.d） or ARB 40 mg/（kg-d） just before the beginning of reperfusion and after reperfusion. The nerve function, changes of infarction volume to whole cerebral volume, brain tissue water content were evaluated. Results Compared with ischemia group, the cerebral infarction volume in EPO-treated group was smaller, and the cerebral water content also decreased, nerve function improved, there were significant differences between the two groups （ P 〈 0. 05 ）. Compared with ischemia group, the cerebral infarction volume in ARB-treated group was smal- ler, and the cerebral water content also decreased, there were significant differences between the two groups （P 〈 0. 05 ）. Conclusion Erythropoietin and angiotensin receptor blocker can decrease the infarction volume, the cerebral water content,improve nerve function, by erythropoietin and angiotensin receptor blocker preconditioning, the result is more well, and has protective effects on ischemia-reperfusion.