血清白三烯B4及C反应蛋白在慢性阻塞性肺疾病中的作用研究

EFFECT OF SERUM LEUKOTRIENE B4 AND C-REACTIVE PROTEIN ON CHRONIC OBSTRUCTIVE PULMONARY DISEASE

目的观察慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者血清中白三烯B4(1eukotriene B4,LTB4)、C反应蛋白(C-reactive protein,CRP)的水平变化,探讨其在COPD发病机制中的作用。方法分别取COPD急性加重期(acute exacerbation of chronic obstructive pulmonary disease,AECOPD)、稳定期(stable chronic obstructive pulmonary disease,SCOPD)患者及健康者空腹静脉血,离心获得血清,采用双抗体夹心ELISA法检测各组血清的LTB4水平,采用散射比浊法检测各组CRP水平。结果 AECOPD和SCOPD患者血清LTB4水平分别是(143.84±20.21)ng/L和(43.82±10.45)ng/L,均显著高于健康者的(13.33±1.71)ng/L(P<0.01);AECOPD和SCOPD患者血清CRP水平分别是(43.77±7.48)mg/L和(11.10±1.34)mg/L,均显著高于健康者的(4.92±1.43)mg/L(P<0.01)。结论 AECOPD患者血清中LTB4、CRP水平明显高于SCOPD患者,SCOPD患者又高于健康者,说明LTB4、CRP参与了COPD的炎症反应,可作为判断疾病严重程度和评估预后的辅助指标。

Objective To observe the change of leukotriene B4 （LTB4） and C-reactive protein （CRP） levels in serum of patients with chronic obstructive pulmonary disease （COPD） and explore their role in pathogenesis. Methods Fasting venous blood samples were collected from acute exacerbations of COPD （AECOPD） patients, stable COPD （SCOPD） patients and healthy people. The samples were centrifuged to obtain serum. Avidinbiotincomplex-EL[SA （ABC-ELISA） method was used to detect serum LTB4 levels and nephelometry Was used to detect serum CRP levels. Results The serum LTB4 level of patients with AECOPD and SCOPD were （ 143.84 ±20.21 ） ng/L and （43.82 ±10.45） ng/L, were significantly higher than that of control （13.33 ±1. 71 ）ng/L（P 〈 0.01 ）;The serum CRP levels of patients with AECOPD and SCOPD （43.77 ±7.48）mg/L and （11.10 ±1.34）mg/L, were significantly higher than that of control （4.92 ±1.43）mg/L（P〈0.01）. Conclusion The LTB4 and CRP levels in serum of patients with AECOPD were significantly higher than those of SCOPD patients, and the LTB4 and CRP levels of SCOPD patients were higher than those of healthy subjects. LTB4 and CRP were involved in the inflammatory response to COPD, which can be used as auxiliary indicators to judge the severity of disease and evaluate the prognosis.