那可丁对人卵巢癌SKOV3细胞增殖和凋亡的影响

EFFECT OF NARCOTINE ON PROLIFERATION AND APOPTOSIS IN HUMAN OVARIAN CANCER SKOV3 CELLS

目的探讨那可丁对人卵巢癌SKOV3细胞体外增殖和凋亡的影响及其可能的作用机制。方法体外培养卵巢癌SKOV3细胞,应用四甲基偶氮唑蓝法检测那可丁对细胞的增殖抑制情况;倒置显微镜观察那可丁对细胞形态的影响;流式细胞术检测细胞凋亡率、细胞周期及细胞中成纤维细胞生长因子2(fibroblast growth factor-2,FGF-2)蛋白的表达情况。结果在一定剂量范围内,那可丁能显著抑制SKOV3细胞的增殖,并呈时间和浓度依赖性(P<0.05)。那可丁作用SKOV3细胞后,细胞出现皱缩、破裂、脱落等凋亡形态学变化。与对照组相比,那可丁可增加SKOV3细胞的凋亡率以及G_2/M期细胞的比例(P<0.05),并降低FGF-2蛋白的表达水平(P<0.05)。结论那可丁可明显抑制人卵巢癌SKOV3细胞的增殖并诱导其凋亡,其作用机制可能与SKOV3细胞G_2/M期阻滞及FGF-2蛋白表达下调有关。

Objective To investigate the effects of narcotine on proliferation and apoptosis of human ovarian cancer SKOV3 cells, and to analyze its possible mechanisms. Methods Human ovarian cancer SKOV3 cells were cultured in vitro, methyl thiazolyl tetrazolium method was applied to examine the drug inhibition rate on cell proliferation. Inverted microscope was used to observe cell morphology. Flow cytometry was used to detect cell cycle and apoptosis rate, and the expression of fibroblast growth factor-2 （FGF-2） protein. Results The proliferation of SKOV3 cells was significantly inhibited by narcotine, and in a certain range, the effect was in a time-and dose-dependent manner （P 〈 0.05 ）. The typical apoptotic morphological changes including the shrinkage, cracking, abscission and so on were observed by inverted microscope after narcotine treatment on SKOV3 cells. Compared with the control group,the apoptosis rate and the number of SKOV3 cells in G2/M phase increased significantly （ P 〈 0.05 ） , and the expression of FGF-2 decreased significantly in narcotine treatment group （P 〈 0. 05 ）. Conclusion Narcotine shows an enhanced ability to inhibit the proliferation of human ovarian cancer SKOV3 cells and increase their apoptosis. This effect may be associated with cell cycle G2/M arrest and down-regulation of FGF-2 expression.