固相萃取-超高效液相色谱法同时测定人参中人参皂苷Rg1、Re、Rb1的含量

Simultaneous determination of ginseng Rg1、Re、Rb1 contents in ginseng by ultra performance liquid chromatography

目的建立同时检测人参中人参皂苷Rgl、Re、Rbl的超高效液相色谱（UPLC）分析方法。方法采用wa．tersAcquityUPLCBEHC18色谱柱（1．7μm，50mm×2．1mm）、乙腈一水为流动相、柱温30％、检测波长203nm的色谱条件，同时分离测定人参皂苷Rgl、Re、Rbl。样品以甲醇为提取溶剂，经大孔吸附树脂柱除去基质干扰，取2μl注入UPLC。结果人参皂苷Rgl、Re、Rbl在10—100mg／L范围内呈良好的线性关系，加标回收率在92．1％。105．2％，所测定的相对标准偏差在2．02％～3．85％之间。结论该方法灵敏、快速，结果准确可靠，可为人参中人参皂苷检测提供可靠的分析方法。

Objective To develop an ultra performance liquid chromatographic （UPLC） method for the simultaneous de- termination of ginseng Rgl ,Re,Rbl contents in ginseng. Methods The extracts were separated on an Acquity UPLC BEH Cls （ 1.7μm,50 mm ×2. 1 mm） by mobile phase of acetonitrile - water with UV detector at a wavelength of 203 nm. The an- alytes in ginseng were extracted with methanol. The extract was cleaned up using resin cartridge. Injection volume was 2 μl. Results The linearity of ginseng Rgl, Re and Rbl contents was good in the range of 10 - 100 mg/L. The recovery of the method was 92. 1% - 105.2% , and relative standard deviation（RSD） was 2. 02% -3. 85%. Conclusion The method is accurate, quick and reliable.