八珍袋泡茶中阿魏酸的含量测定

Determination of Ferulic acid in Bazhen Daipaocha by HPLC

目的：建立HPLC测定八珍袋泡茶中阿魏酸的含量。方法：采用C18色谱柱（250mm×4．6mm，5μm），流动相为乙腈-0．085％磷酸溶液（17：83），流速为1．0mL·min^-1，检测波长为313nm。结果：阿魏酸进样量在0．41～2．46μg线性关系良好（r=0．9998），平均回收率为99．02％，RSD为0．39％（n=9）。结论：此方法简便、准确，可用于八珍袋泡茶中阿魏酸的含量测定。

Objective： To develop an HPLC method for determination of Ferulic acid in Bazhen Daipaocha. Methods： The column was Diamonsil C18 （250mm×4.6mm,5μm）, acetonitrile-phosphoric acid （17： 83） as mobile phase at a flow rate of 1. 0 mL· min^ -1 and the detecting wavelength was set at 313 nm. Results： There was a good linear relationship for Ferulic acid within the range of 0. 41 - 2. 46 μg. The average recovery rate was 99.02% and RSD was 0.39% （n =9）. Conclusion： The method is simple, quick and can be used to determin the Ferulic acid of Bazhen Daipaocha.