植物蛋白胨在肺炎链球菌培养基中的应用

The application of phytone in the pneumococcal culture medium

目的 用植物蛋白胨替代目前肺炎链球菌（pneumococcus,Pn）培养基中的动物蛋白胨,以保证生物制品的安全性.方法 比较大豆蛋白胨与胰蛋白胨的各种成分含量.分别以大豆蛋白胨和胰蛋白胨制备培养基进行9个型Pn大罐培养,比较两种培养基培养对Pn生长和粗制多糖含量的影响.结果 大豆蛋白胨的总氮含量、氨氮含量和消化系数与胰蛋白胨相近,但大豆蛋白胨的总碳水化合物含量（336.20 μg/g）明显高于胰蛋白胨的总碳水化合物含量（10.56 μg/g）.植物源性培养基培养获得的各型Pn浓度（t=3.851,P＜0.05）和粗制多糖含量（t=3.853,P＜0.05）明显高于动物源性培养基培养.结论 植物蛋白胨可替代动物蛋白胨来制备Pn培养基.

Objective To replace animal peptone by phytone in the pneumococcal culture medium in order to ensure the safety of biological products.Methods Contents of various components in soy peptone were compared with those in animal peptone.Two kinds of pneumococcal culture media were prepared by using soy peptone and tryptone respectively to culture 9 types of pneumococcus in the large fermentation tank.The influence of 2 kinds of pneumococcal culture media on pneumococcal growth and content of crude polysaccharide were compared.Results The total nitrogen content,ammonia-nitrogen content and digestion coefficient of soy peptone were similar to tryptone,but the total carbohydrate content of soy peptone （336.20 μg/g） was significantly higher than that of tryptone （10.56 μg/g）.The concentrations （t =3.851,P ＜ 0.05） and the contents of crude polysaccharide （t =3.853,P ＜ 0.05） of different types of pneumococcus harvested from the plant-derived culture media were significantly higher than those harvested from the animalderived culture media.Conclusion Animal peptone can be substituted by phytone to prepare the pneumococcal culture medium.