期刊文献+

多药耐药鲍曼不动杆菌AdeB外排系统与碳青霉烯类耐药的关系 被引量:1

The relationship between AdeB efflux system of multidrug resistant Acinetobacter baumannii and carbapenem resistance
原文传递
导出
摘要 目的 探讨我国多药耐药鲍曼不动杆菌(鲍氏不动杆菌,MDR-AB)AdeB外排系统对碳青霉烯类抗生素耐药的介导作用.方法 选取全国21家医院MDR-AB菌株101株和敏感菌株12株,用琼脂稀释法检测外排泵抑制剂苯丙氨酸-精氨酸-β-苯胺(PAβN,50 mg/L)对菌株AdeB外排系统的抑制作用及菌株对亚胺培南和美罗培南的耐药情况.利用实时荧光定量PCR技术分析adeB的表达情况,并探讨其与外排表型及耐药之间的关系.结果 101株MDR-AB对亚胺培南和美罗培南的耐药率分别为62.4%和52.5%.1 13株菌株中,共有82株adeB阳性菌株,其中adeB表达比率>2.5的7株菌株均表现为多重耐药.亚胺培南的耐药组和非耐药组adeB的表达比率分别为1.237±0.159和0.698±0.115,差异有统计学意义(t=2.641,P<0.05);而美罗培南耐药组和非耐药组adeB的表达比率分别为1.013±0.148和0.978±0.150,组间差异无统计学意义(t=0.166,P>0.05).结论 我国MDR-AB中,AdeB外排泵系统的过度表达介导了其对亚胺培南耐药,并可能和美罗培南耐药有关. Objective To investigate the role of AdeB efflux system of multidrug resistant Acinetobacter baumannii (MDR-AB) in carbapenem resistance.Methods One hundred and one isolates of MDR-AB and 12 isolates of antibiotic susceptible Acinetobacter baumannii were collected from 21 hospitals in China.Agar dilution method was used to evaluate the inhibitory effect of efflux pump inhibitor phenyl-arginine-β-naphthylamide (PAβN,50 mg/L) on AdeB-mediated efflux and the drug resistance of MDR-AB to imipenem and meropenem.Real time-PCR was used to determine the expression of adeB gene.Results The resistance rates of 101 MDR-AB isolates to imipenem and meropenem were 62.4% and 52.5%,respectively.Of 113 isolates,82 were adeB gene positive,7 of which showed significantly high adeB expression (expression rate 〉2.5) and were all MDR-AB.The ratio of adeB expression was significantly different between resistant isolates (1.237±0.159) and susceptible isolates (0.698±0.115) of imipenem (t=2.641,P〈0.05),but no significant difference was noted between resistant isolates (1.013±0.148) and susceptible isolates (0.978±0.150) of meropenem (t=0.166,P〉0.05).Conclusions The over expression of AdeB efflux pump may play a role in imipenem resistance in MDR-AB but its effect on meropenem resistance is uncertain.
出处 《国际流行病学传染病学杂志》 CAS 2014年第1期21-24,共4页 International Journal of Epidemiology and Infectious Disease
基金 国家自然科学基金(81000757) 杭州市卫生科技计划(2011B003) 杭州市科技发展计划(20110833B27)
关键词 鲍氏不动杆菌 外排系统 AdeABC 实时荧光定量PCR 碳青霉烯类 Acinetobacter baumannii Efflux system AdeABC Real-time PCR Carbapenems
  • 相关文献

参考文献13

  • 1Peleg AY,Seifert H,Paterson DL.Acinetobacter baumannii:emergence of a successful pathogen[J].Clin Microbiol Rcv,2008,21:538-582.
  • 2Anstey NM,Currie B J,Withnall KM.Community-acquired Acinetobacter pneumonia in the Northern Territory of Australia[J].Clin Infect Dis,1992,14:83-91.
  • 3Perez F,Hujer AM,Hujer KM,et al.Global challenge of multidrug-resistant Acinetobacterbaumannii[J].Antimicrob Agents Chemother,2007,51:3471-3484.
  • 4Marchand I,Damier-Piolle L,Courvalin P,et al.Expression of the RND-type efflux pump AdeABC in Acinetobacter baumannii is regulated by the AdeRS two-component system[J].Antimicrob Agents Chemother,2004,48:3298-3304.
  • 5Hu WS,Yao SM,Fung CP,et al.An OXA-66/OXA-51-1ike carbapenemase and possibly an efflux pump are associated with resistance to imipenem in Acinetobacter baumannii[J].Antimicrob Agents Chemother,2007,51:3844-3852.
  • 6Huang L,Sun L,Xu G,et al.Differential susceptibility to carbapenems due to the AdeABC efflux pump among nosocomial outbreak isolates of Acinetobacter baumannii in a Chinese hospital[J].Diagn Microbiol Infect Dis,2008,62:326-332.
  • 7Cetinkaya E,Coban AY,Durupinar B.Investigation of the effect of efflux pump inhibitors to MIC values of ciprofloxacin in clinical isolates of Pseudomonas aeruginosa,Escherichia coli,Acinetobacter baumannii and Staphylococcus aureus[J].Mikrobiyol Bul,2008,42:553-561.
  • 8Donald HM,Scaife W,Amyes SG,et al.Sequence analysis of ARI-1,a novel OXA beta-lactamase,responsible for imipenem resistance in Acinetobacterbaumannii6B92[J].Antimicrob Agents Chemother,2000,4:196-199.
  • 9Su XZ,Chen J,Mizushima T,et al.AbeM,an H +-coupled Acinetobacter baumannii multidrug efflux pump belonging to the MATE family of transporters[J].Antimicrob Agents Chemother,2005,49:4362-4364.
  • 10Chu YW,Chau SL,Houang ET.Presence of active efflux systems AdeABC,AdeDE and AdeXYZ in different Acinetobacter genomic DNA groups[J].J Med Microbiol,2006,55(Pt 4):477-478.

同被引文献7

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部