热休克蛋白70基因多态性在冠心病中的作用

Role of heat shock protein 70 gene polymorphism on coronary heart disease

目的:探讨热休克蛋白(HSP)70-1(+190G/C)、HSP70-2(+1 267A/C)和HSP70-hom(+2 437T/C)的基因多态性在冠心病中的作用。方法:选择237例冠心病和203例非冠心病患者,均无血缘关系。采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)方法检测HSP70-1基因+190G/C、HSP70-2基因+1 267A/G、HSP70-hom基因+2 437T/C多态性。结果:研究人群中存在3种基因的多态性,符合HardWeiberg遗传平衡分布。HSP70-1基因C等位基因和HSP70-2基因G等位基因在两组中的分布差异有统计学差异(χ2=13.781,P<0.005和χ2=7.577,0.01<P<0.025)。HSP70-hom基因T等位基因在两组中的分布无统计学差异(χ2=0.343,0.75<P<0.9)。Logistic多元回归分析得出HSP70-1基因(GC+CC)基因型和HSP70-2基因(AG+GG)基因型均是冠心病的独立危险因素,而HSP70-hom基因则无。结论:HSP70-2基因+1 267A/G和HSP70-1基因+190G/C多态性与冠心病的危险性有关联。其中(GC+CC)基因型、C等位基因和(AG+GG)基因型、G等位基因分别是冠心病的独立危险因素。HSP70-hom基因+2 437T/C多态性与冠心病的危险性关联不大。

Objective：To investigate the role of HSP70-1, HSP70-2, HSP-hom gene polymorphism on CHD. Method：The 237 patients with CHD （CHD group） and 203 people with non CHD （control group） were collected in the study. We detected the polymorphism of HSP 70-1 gene ＋190 G/C site, HSP 70-2 gene ＋1 267 A/G site, HSP70-hom gene ＋2 437 T/C site by polymerase chain reaction-restriction fragment length polymorphism （PCR- RFLP）. Result：There were three genes polymorphism found in this study population. The distribution of these genotypes was in the Hardy-Weinberg equilibrium. The prevalence of HSP70-1 gene C allele HSPT0-2 gene G allele in CHD group was statistically significantly higher than in control subjects （X2 = 13. 781, P〈0. 005; X2 = 7. 577, 0. 01〈P〈0. 025）. The prevalence of HSP70-hom gene T allele in CHD group was not statistically significantly higher than in control subjects （X2 =0. 343, 0. 75〈P〈0. 9）. Multi-factors stepwise logistic regression analysis showed The HSPT0-1 （GC＋CC） genotype and the HSP70-2 （AG＋GG） genotype are independent risk factors of CHD, While the HSP70-hom gene is not. Conclusion：The HSP70-1 ＋90 G/C and HSP70-2 ＋1 267 A/G gene polymorphism are associated with the risk of CHD. The （GC＋CC） genotype and C allele, the （AG＋GG） genotype and G allele are independent risk factors of CHD. While the HSP70-hom gene ＋2 437 T/C polymorphism is not.