果糖和胰岛素对鹅胚原代肝细胞硬脂酰辅酶A去饱和酶1基因表达量的影响

Effects of Fructose and Insulin on Stearyl-Coenzyme A Desaturase 1 Gene Expression Level in Primary Germinal Hepatocytes of Geese

本试验旨在研究果糖和胰岛素对鹅胚原代肝细胞硬脂酰辅酶A去饱和酶1(SCD1)基因表达量的影响。以鹅胚原代肝细胞为试验对象,研究不同浓度果糖(0、10、30、50 mmol/L)、胰岛素(0、50、100、200 nmol/L)以及两者协同作用(10 mmol/L果糖+50 nmol/L胰岛素、10 mmol/L果糖+200 nmol/L胰岛素、50 mmol/L果糖+50 nmol/L胰岛素、50 mmol/L果糖+200 nmol/L胰岛素)对鹅胚原代肝细胞SCD1基因表达量的影响。结果表明:10 mmol/L果糖能够极显著上调鹅胚原代肝细胞SCD1基因表达量(P<0.01),30、50、100 mmol/L果糖对SCD1基因表达量均无显著影响(P>0.05);200 nmol/L胰岛素能够显著上调SCD1基因表达量(P<0.05);10 mmol/L果糖和200 nmol/L胰岛素协同作用能极显著上调SCD1基因表达量(P<0.01)。结果提示,适宜的果糖、胰岛素单独添加以及协同作用均能上调鹅胚原代肝细胞SCD1基因表达量。

The aim of this study was to detect the effects of fructose and insulin on stearyl-coenzyme A desatu-rase 1 （ SCD1 ） gene expression level in primary germinal hepatocytes of geese.Primary germinal hepatocytes of geese were used as test object, the effects of different concentrations of fructose （ 0 , 10 , 30 and 50 mmol/L）, insulin （0, 50, 100 and 200 nmol/L） and their combination （10 mmol/L fructose ＋50 nmol/L insulin, 10 mmol/L fructose＋200 nmol/L insulin, 50 mmol/L fructose＋50 nmol/L insulin and 50 mmol/L fructose＋200 nmol/L insulin） on the SCD1 gene expression level in primary germinal hepatocytes of geese were detected.The results showed as follows： 10 mmol/L fructose could significantly up-regulate SCD1 gene expression level （ P〈0 .01 ） , and 30 , 50 and 100 mmol/L fructose had no significant effect on it （ P〉0 .05 ）; 200 nmol/L insulin could significantly up-regulate SCD1 gene expression level （ P 〈0 .05 ）;10 mmol/L fructose and 200 nmol/L insulin had significantly synergistic effect on the up-regulation of SCD1 gene expression level.The results indicate that fructose and insulin alone supplementation and the combination at proper levels can up-regulate SCD1 gene expression level in primary germinal hepatocytes of geese.