6株酵母菌产营养活性物质能力的比较分析

Comparative Analysis on the Capacities of Producing Nutricines for Six Yeasts

本试验旨在为后期反刍动物微生态制剂研制筛选出高活性酵母菌株。试验测定了6株酵母菌[产朊假丝酵母(Cadida atilis)BY、热带假丝酵母(Candida tropicalis)BR、热带假丝酵母B2、酿酒酵母(Saccharomyces cerevisiae)YR、酿酒酵母YC和酿酒酵母BC]产营养活性物质(β-葡聚糖、甘露聚糖、有机酸、氨基酸和多肽)的能力,并使用DPS 14.10软件运用Topsis法对试验数据进行了多指标综合分析。结果表明:热带假丝酵母BR产β-葡聚糖、甘露聚糖能力最强,其细胞壁中β-葡聚糖、甘露聚糖含量分别为80.2、60.5 mg/g;产朊假丝酵母BY产有机酸能力最强,其培养液中总有机酸含量为344.46μg/mL;酿酒酵母BC产多肽能力最强,其培养液中多肽含量为14.08 mg/dL。多指标综合分析结果表明最优菌株为热带假丝酵母BR,其产β-葡聚糖、甘露聚糖、丁二酸、谷氨酸和半胱氨酸的能力均最强,其细胞壁中β-葡聚糖、甘露聚糖含量分别为80.2、60.5 mg/g,培养液中总有机酸、总氨基酸含量分别达到312.11μg/mL、4.705 mg/g。通过6株酵母菌产营养活性物质能力综合比较分析得出最优菌株为热带假丝酵母BR。

This experiment was conducted to find out the high activity strains for the development of ruminant microecological agents in latter.The capacities of producing nutricines （β-glucan, mannan, organic acids, a-mino acids and polypeptides） for 6 yeasts （ Cadida atilis BY, Candida tropicalis BR, Candida tropicalis B2 , Saccharomyces cerevisiae YR, Saccharomyces cerevisiae YC and Saccharomyces cerevisiae BC） were deter-mined and the multi-indices synthetical analysis was used to analyze experimental data by Topsis method in DPS 14.10 software.The results showed as follows：the capacities of producingβ-glucan and mannan of Can-dida tropicalis BR were the strongest, and the contents of β-glucan and mannan in cell wall of Candida tropi-calis BR were 80 .2 and 60 .5 mg/g, respectively; the capacity of producing organic acids of Candida utilis BY was the strongest, and the content of total organic acids in culture of Candida utilis BY rearched 344.46 μg/mL;the capacity of producing polypeptides of Saccharomyces cerevisiae BC was the strongest, and the content of polypeptides was 14.08 mg/dL.Multi-indices synthetical analysis results showed that the optimal strain was Candida tropicalis BR, and it had the strongest capacities of producing β-glucan and man-nan, succinic acid, glutamic acid and cysteine.The contents of β-glucan and mannan in cell wall of Candida tropicalis BR were 80.2 and 60.5 mg/g, respectively, and the contents of total organic acids and total anima acids in culture of Candida tropicalis BR were up to 312.11 μg/mL and 4.705 mg/g, respectively.The opti-mal strain with the highest capacities of producing nutricines is Candida tropicalis BR among 6 yeasts by com-prehensive comparative analysis.