高效毛细管电泳测定药品和人体血清中的氢溴酸加兰他敏

Determination of Galanthamine Hydrobromide in Pharmaceuticals and Human Serum by HPCE

利用毛细管区带电泳法测定药品和人体血清中的氢溴酸加兰他敏。最佳实验条件是：50mmol／LNaH2PO4-H3P04，pH=2．5为背景缓冲溶液，分离电压为15kV，温度为25℃，检测波长为289nm。实验结果表明：氢溴酸加兰他敏在0．015-0．160mg／mL范围内呈良好的线性关系，r=0．9989，最低检出限是2．57×10-5mg／mL。日内和日间迁移时间和峰面积的相对标准偏差RSD分别是0．10％～0．22％、0．13％～1．10％和0．14％～0．34％、0．76％～1．63％，精密度和重现性均良好。该方法可简单、快捷地检测药品和人体血清中的氢溴酸加兰他敏。

The complex between Pd2. and 9-Bromophenanthrene （9-BrP） which offers remarkable enhancement to the fluores- cence of 9-BrP was found for the first time. The maximum excitation/emission wavelength of 9-BrP-Pd2＋ are 247 and 364 nm, sepa- rately. According to the fluorescent enhancement theory, the coordination number of 9-BrP-Pd2＋ was calculated to be 3, complex con- stant KA is 2.812× 10 4, the linear correlation coefficient is 0.9983. Pd2＋, when the concentration is in the range of 1 × 10-7 to 2× 10-5mol. L 1, has a well Linear correlation with 9-BrP （R2=0.9962）. The complex of Pd2＋ with 9-BrP shows good selectivity and specifity, while the results of detecting Pd2＋ in tap water sample and the standard addition test are also ideal, indicating the feasibility of using 9-BrP as a fluorescent probe for determination of Pd2＋.