摘要
通过测定卵的直径、卵粒质量、受精率和孵化率系列指标以及精子的激活率、快速运动时间和寿命系列指标对人工驯养子一代中华鲟配子进行质量评价,并通过该批受精卵孵出的子二代系列生长指标进行其发育状况分析。结果显示,子一代所产卵子形态饱满,卵径平均3.71 mm,小于自然繁殖群体。与历年在本基地养殖的自然繁殖群体统计资料相比,其受精率(60.1%)和孵化率(36.8%)处在中等偏下的水平。2012年和2013年的精子活力监测结果发现,子一代精子有效运动时间(漩涡运动+快速运动)均较野生群体高。表明子一代卵子质量较野生群体有所降低,而精子活力则较野生群体高。与历年该基地集约化养殖系统培育出的子一代中华鲟相比,子二代的生长表现出显著的阶段差异性,其中仔鱼阶段生长较慢,稚鱼和幼鱼阶段生长优势则较明显。研究表明,促进人工驯养条件下的子一代后备亲鱼性腺发育完善,以获得较高质量的配子和子二代,对防止中华鲟种质退化至关重要。
The gamete quality of F_1-generation Chinese sturgeon (Acipenser sinensis) was evaluated by a series of biological indexes of eggs ( diameter, weight, fertilization rate and hatching rate ) and sperm ( the highest activation rate, life time and fast movement time). And the growth characteristics of F_2-generation (2012-11 -2013-07)were analyzed by following up the development situation. Results showed that the eggs of F_1-generation were full shape and the average diameter was 3.71 mm, which was less than that of wild population. The fertilization rate(60.1% )and hatching rate (36.8 % )were both at lower-middle level when compared with data over the years. Sperm motility in 2012 and 2013 showed that the effective moving time ( circinate movement time + fast movement time) were longer than those of wild population. It indicated that eggs quality of F_1-generation was lower than that of wild population, and sperm quality was higher yet. In comparison with F_1-generation cultured by intensive farming system in the same aquaculture base over the years,F_2-generation showed obvious growth phase differences. That is,the growth rate was slower in larvae phase but faster obviously in juvenile and young fish phase. Consequently, it was effective to get high quality gamete and F_2-generation by improving the gonad development perfection of cultured F_1-generation in order to prevent the degradation of germplasm. And it was vitally important for Chinese sturgeon species conservation.
出处
《水产学报》
CAS
CSCD
北大核心
2014年第3期350-355,共6页
Journal of Fisheries of China
基金
国家自然科学基金项目(31172413)
农业部淡水生物多样性保护重点实验室开放课题(LFBC0805)
关键词
中华鲟
配子
子代
生长
保护动物
Acipenser sinensis
gamete
generation
growth
animal protection