摘要
采用紫外线照射的方式使精子遗传物质失活,通过细胞松弛素B(CB)的处理来抑制受精卵第二极体的释放,人工诱导紫贻贝(Mytilus gallopovincialis)雌核发育二倍体。将精子在强度为2561μW/(cm^2·s)的紫外线(254 nm)下进行时间梯度的照射,之后与正常的卵子受精,实验发现随照射时间的增加,卵裂率、早期胚胎存活率和D形幼虫发生率总体呈下降趋势,在照射55 s时D形幼虫发生率降为0。受精后25 min,用浓度0.5μg/mL的细胞松弛素B(CB)持续处理受精卵20 min,诱导出紫贻贝第二极体抑制型雌核发育二倍体。通过微卫星分析鉴定所得的子代个体均为雌核发育二倍体。
Gynogenetic diploids in blue mussel Mytilus galloprovincialis were induced by means of ultra- violet （UV） radiation and eytochalasin B （CB） treatment for the inhibition of the second polar body release. The sperms irradiated by UV at an intensity of 2561 μW/（cm^2. s） for various durations were fertilized with the normal eggs. With creasing irradiation time, the cleavage rates, survival rates at ear- ly embryonic phases and developmental rates of D-larvae decreased, and none of D-larvae occurred when the sperms were irradiated for more than 55 s. To induce gynogenetic diploids, 25 min postinsemination, the fertilized eggs were exposed to cytochalasin B treatment （0.5 μg/mL; CB） for 20 min. Microsatellite analysis demonstrated that gynogenetie diploids were successfully induced in M. galloprovincialis.
出处
《海洋湖沼通报》
CSCD
北大核心
2014年第1期42-47,共6页
Transactions of Oceanology and Limnology
基金
国家自然科学基金(31072207)
国家海洋公益性行业科研专项(201305005)
国家科技支撑计划(2011BAD13B01)资助
关键词
紫贻贝
紫外线照射
雌核发育
微卫星
Mytilus galloprovincialis
UV radiation
gynogenesis
microsatellite
