MACC1、c-Met蛋白在宫颈癌中的表达及与盆腔淋巴结转移的关系研究

Expression of MACC1 and c-Met Proteins in Cervical Carcinoma and its Correlation with Pelvic Lymph Node Metastasis

目的：探讨结肠癌转移相关基因1（MACCl）、肝细胞生长因子受体（c-Met）蛋白在宫颈癌中的表达及与盆腔淋巴结转移的关系。方法：采用免疫组织化学检测10例正常宫颈组织、17例中～重度宫颈上皮内瘤变组织（CINⅡ～Ⅲ）和91例宫颈癌组织中MACCl、c．Met蛋白的表达情况，分析两者在宫颈癌组织中表达的相关性及与宫颈癌盆腔淋巴结转移的关系。结果：MACCl、c．Met蛋白在宫颈癌组织、CINⅡ～Ⅲ组织中的阳性表达率分别依次为83．5％、76．5％，80．2％、70．6％，均显著高于正常宫颈组织（P〈0．05）。宫颈癌中MACCl蛋白的表达与c．Met蛋白的表达呈正相关（r=0．672，P〈0．05）。将宫颈癌盆腔淋巴结转移组与无转移组进行单因素比较分析，MACCl蛋白表达、c．Met蛋白表达、组织学分化程度、脉管浸润、肌层浸润深度与宫颈癌盆腔淋巴结转移有关（P〈0．05），进一步Logistic回归分析表明，MACCl蛋白表达、肌层浸润深度是宫颈癌盆腔淋巴结转移的独立危险因素（P〈0．05）。结论：MACC1、c．Met蛋白异常表达可能协同参与宫颈癌的恶性进展，MACCl蛋白高表达是宫颈癌盆腔淋巴结转移的独立危险因素。

Objective： To investigate the expressions of metastasis-associated in colon cancer-1 （ MACC1 ）, and hepatocyte growth factor receptor （c-Met） in cervical carcinoma and their significance. Methods：The expressions of MACC1 and c-Met in 10 specimens of normal cervical tissue, 17 specimens of moderate-Se- vere cervical intraepithelial neoplasia and 91 specimens of cervical carcinoma were measured by immunohis- tochemistry. The correlations between MACC1 and c-Met protein in cervical carcinoma were analyzed, and the correlations of the 2 proteins with pelvic lymph node metastasis were also evaluated. Results ：The posi- tive expression rates in cervical carcinoma and CIN Ⅱ～Ⅲwere 83.5% and 76.5% for MACC1 ; and 80.2% and 70.6% for c-Met; respectively, which were significantly higher than those in normal cervical tissue（ P 〈 0. 05）. MACC1 expression was positively correlated to c-Met expression（ r =0. 672, P 〈 0.05）. The single factor comparative analysis between metastasis group and none-metastasis group showed that the expres- sion of MACC1 and c-Met, histological differentiation, vascular invasion, and muscle invasion depth were as- sociated with cervical carcinoma pelvic lymph node metastasis （ P 〈 0. 05 ）. Logistic regression analysis showed that MACC1 expression and muscle invasion depth were independent risk factors of cervical carcino- ma pelvic lymph node metastasis （P 〈0.05）. Conclusions：Deregulation of MACC1 and c--Met may syner- gistically participate in the malignant progression of cervical carcinoma. The expression of MACC1 is an inde- pendent risk factor of cervical carcinoma pelvic lymph node metastasis.