电针对脑缺血再灌注模型大鼠脑源性神经营养因子表达的影响

The effect of electroacupuncture the expression of brain-derived neurotrophic factor in cerebral ischemiareperfusion injury model rats

目的:观察电针(EA)对脑缺血再灌注模型大鼠脑组织中脑源性神经营养因子(BDNF)表达,探讨电针治疗缺血性脑卒中的作用机制。方法:SPF级雄性大鼠60只,随机分为假手术组、模型组和电针组,每组均为20只;模型组和电针组均用改良的线栓法建立大鼠左侧大脑中动脉缺血(MCAO)再灌注模型。电针患侧"曲池穴"和"足三里穴"30min,1次/d,至动物处死。蛋白免疫印记杂交和聚合酶链反应技术(RT-PCR)检测大鼠左侧皮质BDNF蛋白和基因表达情况。结果:①与假手术组比较,模型组和电针组BDNF蛋白和基因表达升高(P<0.05);②与模型组相比,电针组BDNF蛋白和基因表达均增高P<0.05)。结论:缺血再灌注模型大鼠大脑皮质中的BDNF短暂应激性表达增高,这可能是电针能够增强缺血性脑卒中BDNF的脑保护作用的生物学机制之一。

Objective： To observe the effect of electroacupuncture（EA） on expression of brain-derived neurotrophic factor （BDNF）in the brain of cerebral ischemia reperfusion model rats to study the mechanism of EA treatment for cerebral stroke. Method：Sixty SPF SD male rats were randomly divided into 3 groups： sham operation group, ischemia group, EA group, 20 rats in each group. All groups except sham operation group received left middle cerebral artery occlusion（MCAO）. EA was applied at ipsilateral Quchi（Llll） and Zusanli（ST36）, 30min once a day until the rats sacrificed. The changes of BDNF in left cortex were examined by RT-PCR and Western blot. Result：Compared with sham operation control group, in ischemia group and EA group the gene and protein lev- els of BDNF increased（P〈0.05）. But the gene and protein levels of BDNF in EA group were higher than that in ischemia control group（P〈0.05）. Couclusion：EA at Quchi and Zusanli acupoints of contralateral paralyzed limb in cerebral ischemia reperfusion model rats can enhance the expression of BDNF in cerebral cortex, and it may be one of the biological mechanism of brain protection of EA in cerebral stroke.