壮筋续骨汤对骨折大鼠血清CT和维生素D_3水平的影响和意义

Effect and significance of ZJXG decoction on the serum levels of CT and VitD_3 in femur fracture rats

目的研究壮筋续骨汤对大鼠股骨骨折后降钙素（CT）和活性维生素Ds（VitD3）水平影响。方法成年健康雄性Wistar大鼠72只，切断股骨中段制备骨折模型，采取壮筋续骨汤灌胃治疗。X线观察骨折愈合情况，酶联免疫吸附试验检测血清CT和VitD3水平。结果大鼠骨折后7d骨折线明显，14d纤维性骨痂形成，21d开始钙盐沉着，28d有骨性骨痂形成。大鼠骨折后7～21d，治疗组骨痂结构变化与模型组相应时间比较无明显差异，但至28d治疗组骨痂结构优于模型组。模型组和治疗组大鼠血清CT和VitD3水平在骨折后7～28d明显高于对照组（P〈0．05），模型组与治疗组在7～21d无显著差异，但在28d治疗组明显高于模型组（P〈0．05）。结论壮筋续骨汤可在骨折愈合后期通过维持血清CT和VitD3水平而促进骨折愈合。

Objective To investigate the effects of Zhuang Jin Xu Gu（ZJXG） decoction on the serum levels of calcitonin（CT） and active vitamin D3（VitD3） in femoral fracture rats. Methods Femur fractures were generated in 72 male adult Wistar rats by cutting femur transversely at middle point. ZJXG decoction was administered orally after surgery for 28d. The healing process was analyzed by X-ray. The serum levels of CT and VitD3 were assessed by enzyme linked immunobsorbent assay（ELISA）. Results X-ray indicated that the fracture line of the femoral fracture- end was clear at 7d, fibrous callus tissue formed at 14d, Ca2^＋ salt deposited at 21d and osseous callus tissues formed at 28d. During fracture 7-21d, no difference of the callus tissues structure existed between model and treatment groups, but in treatment group it was better than that in model group at 28d. ELISA results showed that the serum levels of CT and VitD3 were higher than those in control group at 7-28d（P 〈 0.05）. There were no significant difference between model and treatment groups at 7-21d, but in treatment group it was higher than that in model group at 28d（P 〈 0.05）. Conclusion ZJXG decoction could enhance the fracture healing by reducing the decomposition of CT and VitD3 in femur fracture rats.