饮茶型氟中毒病区不同民族人群血清甲状旁腺激素水平及其基因多态性的差异

Differences of serum parathyroid hormone levels and its gene polymorphism in different ethnic groups in drinking brick-tea-borne endemic fluorosis areas

目的探讨饮茶型氟中毒病区不同民族人群血清甲状旁腺激素（PTH）含量及其基因多态性的种族分布差异。方法在内蒙古、青海和新疆的饮茶型氟中毒重点病区乡，对16岁以上人群进行问卷调查，内容包括基本信息、膳食调查和总氟摄人情况．并采集外周静脉血，采用质谱法进行胛H基因单核苷酸多态性（SNP）分型，采用放射免疫法检测血清中PTH含量。结果共有1175位受试者入选，其中蒙古族308位、哈萨克族295位、俄罗斯族155位、藏族381位、汉族36位。受试者全血的PTH基因分为AA．AG、GG基因型，基因分布符合Hardy-weinberg平衡。AA、AG、GG3种基因型分布频率，蒙古族分别为0．6％（2／308）、20．2％（62／308）、79．2％（244／308）；哈萨克族分别为4．4％（13／295）、25．1％（74／295）、70．5％（208／295）；俄罗斯族分别为2．0％（3／155）、33．5％（52／155）、64．5％（100／155）；藏族分别为2．4％（9／381）、31．2％（119／381）、66．4％（253／381）；汉族分别为5．6％（2／36）、13．8％（5／36）、80．6％（29／36）。不同民族之间基因型分布频率比较差异有统计学意义（x2=28．86，P〈0．05）。共检测1009份血清，其中蒙古族245份、哈萨克族282份、俄罗斯族105份、藏族345份、汉族32份，血清PTH含量分别为（16．36±2．48）、（14．30±3．76）、（15．22±3．92）、（13.26±3．66）、（15.42±1．12）mg／L，各民族间比较差异有统计学意义（F=31．25，P〈0．05）。AA、AG和GG3种基因型携带者（分别为27、270和712例）血清明H含量分别为（13．75±2．05）、（14．30±3．80）、（14．67±3．65）mg／L，组间比较，差异无统计学意义（F=1．72，P〉0．05）。结论在饮茶型氟中毒病区不同民族人群血清中PTH含量不同，PTH基因多态性分布存在种族差异，但携带不同PTH基因型人群的血清唧水平未见明显的差别。

Objective Inthis study, the differences of serum parathyroid hormone（PTH） and its gene polymorphism in different ethnic groups in drinking brick-tea-borne endemic fluorosis areas were investigated. Methods Inhabitants over the age of 16 years old in Inner Mongolia, Qinghai and Xinjiang were investigated. The questionnaire survey included basic information, dietary survey and total fluoride intake, and peripheral venous blood was collected. The PTH gene single nucleotide polymorphism （SNP）genotyping was determined using mass spectrometry, and PTH levels in serum were determined by radioimmunoassay. Results In the 1 175 copies.of whole blood samples （308 Mongolians, 295 Kazakhs, 155 Ethnic Russians, 381 Tibetans and 36 Han people）, PTH genes were typed into AA, AG and GG genotypes, and gene distribution met Hardy-weinberg equilibrium.The frequencies of genotypes AA, AG and GG in Mongolian were 0.6% （2/308）, 20.2% （62/308） and 79.2% （244/308）; the frequencies of the three genotypes in kazakh were 4.4% （13/295）, 25.1% （74/295） and 70.5% （208/295）; the frequencies of the three genotypes in Russians were 2＋0% （3/155）, 33.5% （52/155） and 64.5% （100/155）; the frequencies of the three genotypes in Tibetan were 2.4% （9/381）, 31.2% （119/381） and 66.4% （253/381）; the frequencies of genotypes in Han people were 5.6%（2/36）, 13.8%（5/36） and 80.6%（29/36）. The differences of the three genotype frequencies between different ethnic groups were statistically significant（X2 = 28.86, P 〈 0.05）. One thousand and nine peripheral venous blood were detected. The levels of PTH in the five ethnic groups（245, 282, 105, 345 and 32） were （16.36 ±2.48）, （14.30 ± 3.76）, （15.22 ± 3.92）, （13.26 ± 3.66） and （15.42 ±1.12）mg/L, respectively, and the difference between various ethnic groups was statistically significant（F = 31.25, P 〈 0.05）. Serum PTH levels in different genotypes（AA：27, AG：270, GG：712） were （13.75 ±2.05）, （14.30 ± 3.80） and （14.67 ± 3~65）mg/L, and the difference was not statistically significant between the groups （F = t.72, P 〉 0.05）. Conclusion PTH gene polymorphisms have racial differences in different ethnic groups, and serum PTH levels are different, but the levels of serum PTH are not different in people with different genotypes.