氟砷联合对大鼠骨组织Runx相关基因2mRNA表达的影响

Combined effects of fluoride and arsenite on the expression of Runx-related transcription 2 mRNA in bone of rats

目的探讨氟砷联合对大鼠骨组织Runxa相关基因2（Runx2）mRNA表达的影响。方法sD大鼠54只，体质量为（109．71±10．52）g，雌雄各半。采用随机数字表法，按3×3析因设计方法分成9组，分别为对照组[0mg／kg氟化钠（NaF）＋0．0mg／kg亚砷酸钠（NaAs02）]、低氟组（5mg／kgNaF）、高氟组（20mg／kgNaF）、低砷组（2．5mg／kgNaAs02）、高砷组（10．0mg／kgNaAs02）、低氟低砷组（5mg／kgNaF＋2．5mg／kgNaAs02）、高氟低砷组（20mg／kgNaF＋2．5mg／kgNaAs02）、低氟高砷组（5mg／kgNaF＋10．0mg／kgNaAs02）、高氟高砷组（20mg／kgNaF＋10．0mg／kgNaAsO：），每组6只，雌雄各半，灌胃染毒6个月。实验结束时提取大鼠股骨组织总RNA。实时荧光定量RT—PCR法检测Runx2mRNA的表达。结果对照组、低氟组、高氟组、低砷组、高砷组、低氟低砷组、低氟高砷组、高氟低砷组、高氟高砷组Runx2mRNA表达量分别为1．024±0．015、1．377±0．014、1．587±0．012、1．182±0．015、1．343±0．010、1．444±0．019、1．504±0．013、1．608±0．013、1．714±0．009。实验各组Runx2mRNA表达量均高于对照组（P均〈0．05），Runx2mRNA表达量随染氟、砷剂量增高而增加，存在剂量．效应关系（P均〈0．01）。析因分析结果表明，氟、砷单独作用时可影响Runx2mRNA的表达水平（F值分别为46．967、8．317，P均〈0．05），且二者具有交互作用（F=105．271，P〈0．01）。结论氟或砷单独作用能够促进大鼠骨组织Runx2mRNA表达，且二者具有交互作用。

Objective To explore the combined effects of fluoride and arsenite on the expression of Runxrelated transcription 2 （Runx2） mRNA in bone of Sprague Dawley（SD） rats. Methods Fifty four SD rats were selected [body mass （109.71 ±10.52）g, half male and half female ]. 3 × 3 Factorial experimental design was used to evaluate the combined effects of fluoride and arsenite on ihe expression of Runx2 mRNA by random number talbe. Rats were exposed to NaF, NaAsO2 and NaF plus NaAsO2 for 6 months by oral perfusion at gradient doses, respectively： the control group（0 mg/kg NaF ＋ 0.0 mg/kg NaAsO2）, the low fluoride group（5 mg/kg NaF）, the high fluoride group （20 mg/kg NaF）, the low arsenite group （2.5 mg/kg NaAsO2）, the high arsenite group （ 10.0 mg/kg NaAsO2）, the low fluoride low arsenite group （5 mg/kg NaF ＋ 2.5 mg/kg NaAsO2）, the high fluoride low arsenite group（20 mg/kg NaF ＋ 2.5 mg/kg NaAsO2）, the low fluoride high arsenite group（5 mg/kg NaF ＋ 10.0 mg/kg NaAsO2） and the high fluoride high arsenite group（20 mg/kg NaF ＋ 10.0 mg/kg NaAsO2）. The expression of Runx2 mRNA was determined by quantitative real-time RT-PCR. Results The expressions of Runx2 mRNA in the control, low fluoride, high fluoride, low arsenite, high arsenite, low fluoride low arsenite, low fluoride high arsenite, high fluoride low arsenite and high fluoride high arsenite groups were 1.024 ± 0.015, 1.377 ± 0.014, 1.587 ± 0.012, 1.182 ± 0.015, 1.343 ± 0.010, 1.444± 0.019, 1.504 ± 0.013, 1.608 ± 0.013 and 1.714± 0.009, respectively. The expressions of Runx2 mRNA in experimental groups were higher than those in control group（all P 〈 0.05）, fluoride and arsenite were positively correlated with the expression of Runx2 mRNA （all P 〈 0.01 ）, and there was a dose-response relationship between Runx2 mRNA and fluoride-arsenite levels. Factorial analysis showed that fluorine or arsenic alone could affect the expression level of Runx2（F = 46.967, 8.317, all P 〈 0.05）, and there was a interaction between fluorine and arsenic to the expression of Runx2 mRNA （F = 105.271, P 〈 0.01 ）. Corlelusion Fluoride or arsenic could promote the expression of Runx2 mRNA in bone of rats; there i-s an interaction between fluorine and arsenic to the expression of Runx2 mRNA.