丙泊酚对急性高眼压大鼠视神经保护作用研究

An experimental study on the protective effect of propofol on optic nerve in rat acute ocular hypertention model

目的探讨丙泊酚对急性高眼压大鼠视网膜神经节细胞（retinaganglion cells，RGCs）的保护作用。方法动物实验研究。于2011年3月至2012年4月将35只大鼠随机分为3组：正常组、对照组、丙泊酚组。对照组和丙泊酚组均制备急性高眼压模型，于2h、48h和72h对照组给予生理盐水0．2ml腹腔注射，丙泊酚组则给予丙泊酚0．2ml（100mg／ml）腹腔注射。HE染色后光镜下观察视网膜形态学变化和视网膜神经节细胞计数并免疫组化方法检测小胶质细胞和TNF-α的表达。结果48h、72h对照组的RGCs数低于丙泊酚组，并两组均较正常组低差异有统计学意义（P〈0．05）。2h丙泊酚组TNF—α平均光密度值较正常组差异无统计学意义（P〉0．05），48h、72h则丙泊酚组TNF一仅表达高于正常组差异有统计学意义（P〈0．05）。2h、48h、72h丙泊酚组的TNF-α表达较对照组均低差异有统计学意义（P〈0．05）。2h丙泊酚组视网膜小胶质细胞表达较正常组差异无统计学意义（P〉0．05），48h、72h则丙泊酚组视网膜小胶质细胞表达高于正常组差异有统计学意义（P〈0．05）。2h、48h、72h丙泊酚组视网膜小胶质细胞平均光密度值均低于对照组差异有统计学意义（P〈0．05）。结论小胶质细胞及TNF-α参与了急性高眼压大鼠的视神经损害过程，丙泊酚能降低急性高眼压大鼠小胶质细胞及TNF-α的表达，从而起到对视神经节细胞的保护作用。

Objective To observe the protective effect of propofol on rat retinal ganglion cells （RGCs） in acute ocular hypertention models. Methods Thirty-five rats were randomly divided into 3 groups： normal group, control group, propofol group. Control group and propofol group were made acute ocular hypertention models. Inject propofol 0.2ml （100mg/ml） after being sterilized intra- peritoneally and respectively into the propofol group after making acute ocular hypertention models 2h, 48h and 72h of the right eyes. That was 20mg/mice. Inject saline 0.2ml of being sterilized intra- peritoneally into the control group correspondly. Then took out the full eye and the intraorbital optic nerve for HE staining under the light microscope to observe the morphological changes of the retina and the count of retinal ganglion cells, and for the analysis of microglia cells and the expression of TNF-ct in the retinal layers with immunohistochemically stained. Results The number of retinal gan- glion cells was lower in the control groups of 48 hours and 72 hours than that in the corresponding time points in propofol group, And there was lower than that in the corresponding time points in normal group. In addition to that there was no significant difference among the mean optical density of TNF-α in the propofol group of 2 hours than that in normal group （P 〉0.05）, the average optical density of TNF-αin propofol group of 48 hours and 72 hours were more than that in normal group and there were significantly different （P 〈0.05）. The average optical density of retinal TNF-αwere lower in the propofol group of 2 hours, 48 hours, 72 hours than that in control group of the corre- sponding time points, and there was a significant difference （P 〈0.05）. In addition there was no sig- nificant difference among the mean optical density of retinal microglia in the propofol group of 2 hours than that in normal group （P 〉0.05）. The average optical density of retinal microglia in propo- fol group of 48 hours and 72 hours were more than that in normal group and there were significant- ly different （P 〈0.05）. The average optical density of retinal microglia were lower in the propofol group of 2 hours, 48 hours, 72 hours than that in control group of the corresponding time points, and there was a significant difference （P 〈0.05）. Conclusions Microglia cells and tumor necrosis factor-αinvolves in the damage of optic nerve of the model rat under acute ocular hypertension. In- traperitoneal injection of propofol can reduce the activation of retinal microglia and the level of TNF-α in rat model of acute intraocular pressure, which has the protective function on the retinal ganglion cells of acute ocular hypertention models.