G蛋白β3亚单位基因C825T多态性与高血压维医分型的关系

C825T Polymorphism of G Protein beta3 Subunit Gene and Uygur Hilit Type of Essential Hypertension: a Correlation Study

目的探讨G蛋白β3亚单位(GNB3)基因C825T多态性与新疆维吾尔族人群原发性高血压(essential hypertension,EH)及其维吾尔医学(维医)体液分型之间的关系。方法按维医体液分型标准将EH患者(高血压组)和非高血压病人群(对照组)分为4种体液型,采用多聚酶链式反应-限制性内切酶片段长度多态性分析方法(PCR-RFLP),对新疆维吾尔族不同异常体液型的161例EH患者和379名对照组人群的GNB3基因多态性进行检测,探讨两组间基因型和等位基因分布频率的差异与高血压发病的关系。结果 (1)在新疆维吾尔族人群中GNB3基因C825T多态频率分布符合Hardy-Weinberg平衡(χ2=0.871,P=0.647);(2)GNB3基因C825T位点3种基因型和2种等位基因分布频率在高血压组和对照组间差异无统计学意义(P>0.05);(3)GNB3基因C825T位点3种基因型分布频率在异常胆液质和非异常胆液质型(异常黑胆质、异常血液质、异常黏液质之和)间的差异有统计学意义(χ2=6.905,P=0.032),尤其是异常胆液质型和异常黏液质型间差异最为明显(χ2=10.404,P=0.006),而等位基因分布频率在两组间的差异无统计学意义(P>0.05);(4)161例EH患者中GNB3基因C825T位点3种基因型和2种等位基因分布频率在异常胆液质型与非异常胆液质型间差异有统计学意义(χ2=9.034,P=0.011;χ2=4.701,P=0.03)。结论GNB3基因C825T位点TT基因型和T等位基因可能与新疆维吾尔族人群EH无关,却与非异常胆液质型EH的发病有关,提示维医不同体液分型高血压的发病机制可能存在差异。

Objective To explore the association between C825T polymorphism of G protein be- ta3 subunit （GNB3） gene and different Hilit types of essential hypertension （EH） in the Uygur nationality of Xinjiang. Methods According to Uygur medical theories, EH patients （as the EH group） and non-EH patients （as the control group） were assigned to four Hilit groups. The C825T polymorphism of GNB3 was detected in 161 EH patients and 379 non-EH subjects of different Hilit types by using polymerase chain re- action-restriction fragment length polymorphism （PCR-RFLP） to explore the difference of the genotypes and allelic frequencies and hypertension. Results （1） In Xinjiang Uygur population, the distribution fre- quencies of GNB3 C825T polymorphism were in accordance with Hardy-Weinberg （X2 = 0.871 ,P = 0.647）. （2） There was no statistical difference in the distribution frequencies of three genotypes and two alleles of GNB3 between the EH group and the control group （P 〉0.05）. （3） There was statistical differ- ence in distribution frequencies of three genotypes between the abnormal Sapra and non-abnormal Sapra group （the sum of abnormal Sewda, abnormal Kan, and abnormal Balhem） （X2 =6.905,P =0.032）, es- pecially between the abnormal Sapra and abnormal Balhem groups （X2 =10.404,P =0.006）, but there was no statistical difference in distribution frequencies of alleles between the two groups （P 〉0.05）. （4） In 161 EH patients, there was statistical difference in the distribution frequencies of three genotypes and two alleles between the abnormal Sapra and non-abnormal Sapra group （X2 = 9.034,P =0.011;X2 = 4.701 ,P =0.03）. Conclusions Both IF genotype and T allele of GNB3 C825T polymorphism might not be associated with EH patients in Xinjiang Uygur populations. However, they were correlated with hyper- tension patients of non-abnormal Sapra, indicating the pathogeneses of EH with different Hilit types might be different.