PPARα转基因对小鼠的生理、生化等影响研究

The impact of PPARα gene on the physiology,biochemistry of mouse

目的通过与正常C57BL/6小鼠作对照,在不同月龄分析PPARα转基因小鼠生理、生化等数据,比较其差异性,观察PPARα基因对小鼠的生理、生化是否会产生影响。丰富转基因小鼠数据库内容,为该模型实际应用于临床前药物评价提供理论依据。方法实验分为4组,分别为PPARα雄性组、PPARα雌性组、C57BL/6雄性组和C57BL/6雌性组,9只/组。在动物10周龄、14周龄、18周龄、22周龄、34周龄分别检测血常规、血生化指标,在动物26周龄时观察其心、肝、肾脏器病变情况,并观察动物的一般生长情况。结果 PPARα转基因小鼠与C57BL/6小鼠比较①体重:6周龄到23周龄的PPARα转基因小鼠体重与C57BL/6小鼠体重比较差异无显著性(P>0.05)。②血常规:10周龄PPARα雌性转基因小鼠红细胞计数(RBC)、嗜碱性粒细胞百分比(BAS%)、淋巴细胞百分比(LYM%)明显高于C57BL/6雌性小鼠,差异有显著性(P<0.01)。14周龄PPARα雄性转基因小鼠白细胞计数(WBC)、淋巴细胞百分比(LYM%)明显高于C57BL/6雄性小鼠,差异有显著性(P<0.05)。③血生化:10周龄PPARα雄性小鼠血尿素氮(BUN)、低密度脂蛋白胆固醇(LDL-C)明显高于C57BL/6雄性小鼠,差异有显著性(P<0.05)。14周龄PPARα雄性小鼠天冬门氨酸氨基转移酶(AST)、甘油三酯(TG)明显低于C57BL/6雄性小鼠,差异有显著性(P<0.05)。④组织病理:26周龄PPARα转基因小鼠与C57BL/6小鼠比较,心脏无明显的改变,但肝细胞出现透明病变,肾小管上皮细胞出现脂肪变性。结论系统收集了PPARα转基因小鼠的体重、血常规、血生化、病理等背景资料,成功分析了其与野生型C57BL/6小鼠之间的差异,为该模型的应用提供参考数据。

Objective To confirm whether the gene of PPARα imposes influences on the physiology, biochemistry index of mouse. The index of PPARα transgenic mouse is collected, analysed and the difference is obstained. The database content of transgenic mouse is enriched to provide the theoretical basis that can make the animal model apply in the preclinical drug evaluation. Methods The experiment animals were divided into 4 groups： PPARα male group （PPARa 8 ）, PPARot female group （ PPARα ♀ ）, C57BL/6 male group （ C57BL/6♂ ）, C57BL/6 female group （ C57BL/6 2 ）. When the mice were at the age of 10 weeks, 14weeks, 18weeks, 22weeks, 34weeks, the blood routine , blood biochemistry of them were detected. At the age of 26 weeks, the pathological changes of heart, liver, kidney were detected. At the same time , the growth of them were observed. Results PPARot transgenic mice were compared with C57BL/6mice ①The weight： When the animals were 6 - 23-week-old , there was no difference between the weight of PPARot transgenic mice and that of C57BL/6 mice（P 〉 0.05）. ②The blood routine ： At the age of 10 weeks, the RBC, BAS%, LYM% of PPARa 9 mice were obviously higher than those of C57BL/6 9 mice （P 〈0. 01 ）. At the age of 14 weeks, the WBC,LYM% of PPARα ♂ mice were obviously higher than those of C57BL/6 ♂ mice （P 〈0.05）. ③The blood biochemical index： At the age of 10 weeks, the BUN,LDL-C of PPARα ♂ mice were obviously higher than those of C57BL/6 ♂ mice （P 〈 0. 05）. At the age of 14 weeks, the AST,TG of PPARα ♂ mice were obviously lower than those of C57BL/6 ♂ mice （P 〈 0. 05）. ④ The histopathology： Compared with C57BL/6 mouse, the heart of PPARa transgenic mice at the age of 26 weeks had no obvious change. The liver cell of them had transparent lesions, and the tubular epithelial cells had steatosis. Conclusion The weight, blood routine , blood biochemistry, pathology date of PPARα transgenic mice were systematically collected. The difference between PPARct transgenic mouse and C57BL/6 mouse was successfully analysed, and the referential date was provided for the application of the model.