碘甲烷对雄性大鼠肝脏氧化损伤的研究

Study on liver oxidative damage in male rats with methyl iodide

目的研究碘甲烷对雄性大鼠肝脏的氧化损伤作用。方法将24只雄性SD大鼠随机分成4组,放入静式染毒罐中,实验组分别给予浓度为650 mg/m3、260 mg/m3、130 mg/m3的碘甲烷,每天吸入染毒4 h,连续染毒一周,对照组给予相同环境条件不染毒。染毒结束后对血清、肝脏组织中超氧化物歧化酶(SOD)和血清中谷胱甘肽S转移酶(GST)的活力进行测定。结果随着碘甲烷浓度的升高,大鼠血清、肝脏组织中SOD活力逐渐降低,高、中、低剂量组与对照组存在统计学差异。血清GST活力随着碘甲烷浓度的升高逐渐升高,实验组与对照组之间存在统计学差异,且低剂量组、中剂量组与高剂量组之间也存在统计学差异。结论碘甲烷暴露对大鼠的肝脏可能存在氧化损伤作用。

Objective To explore the oxidative damage effect of methyl iodide on the liver of male rats. Methods Twenty-four male SD rats were randomly divided into 4 groups, put them into the static exposure tank. Three experimental groups were given the methyl iodide with concentrations of 650mg/m3, 260mg/m3and130mg/m3 respectively for a week period of time. The duration of daily inhalation was 4 hours, The control group was given the same environmental conditions without exposure. At the end of the exposure,the level of superoxide dismutase （SOD） in serum and liver tissue and the activity of glutathione S transferase （GST） in serum were measured. Results With the increase of methyl iodide concentration, the SOD activity in the serum and liver tissue of rats decreased. There was significant difference, between experimental groups and control group. Serum GST activity gradually increased with the increase of the concentration of methyl iodide. There was significant difference between the experimental group and the control group, and there was also significant difference among the low dose group, middle dose group and high dose group in the serum level of GST. Conclusions Methyl iodide exposure may has the effect of oxidative damage on rat liver.