Apocynin对缺血再灌注后急性肾损伤的保护作用

The protective effect of Apocynin on acute renal injury after ischemia/reperfusion

目的观察Apocynin对急性缺血再灌注肾损伤(I/R)大鼠模型的保护作用并探讨其机制。方法采用雄性SD大鼠制备急性缺血再灌注肾损伤模型,用不同浓度(5 mg/kg、10 mg/kg、20 mg/kg)Apocynin干预,同时设定假手术组、手术对照组,生化法测定血肌酐值,光镜下观察肾组织形态学变化并进行肾小管病变计分,免疫组织化学检测肾组织单核巨噬细胞计数,硫代巴比妥酸比色法测定肾组织匀浆丙二醛(MDA),改良DTNB显色法测定血清谷胱甘肽过氧化物酶(GPx),ELISA法测定血清白介素1(IL-1)。结果与假手术组相比,手术对照组血肌酐明显升高(P<0.05),肾小管计分明显升高(P<0.05),肾组织巨噬细胞数目增加(P<0.05),血清MDA、IL-1升高(P<0.05),GPx下降(P<0.05);与手术对照组相比,各Apocynin干预组血肌酐下降(P<0.05),肾小管计分降低(P<0.05),肾组织巨噬细胞数目减少(P<0.05),血清MDA降低(P<0.05),IL-1降低(P<0.05),GPx含量升高(P<0.05)。结论Apocynin可以降低急性缺血再灌注肾组织氧化应激水平,抑制炎症反应,减轻肾功能损伤程度。

Objective To investigate the protective effect of Apocynin on acute renal injury after ischemia/ reperfusion （I/R）. Methods Acute renal ischcmia/reperfusion injury in male SD rats was made by cutting right kidney and clamping left renal artery. SD rats were randomly divided into groups （sham-operated group, I/R group, I/R ＋ Apoeynin groups）. Serum ereatinine was assayed by biochemistry. Renal morphology changes were observed by light microscope. The amount of monocyte were observed by immunohistochemistry. MDA was assayed by thiobarbiturate. GPx was assayed by DTNB. IL-1 was assayed by Enzyme-linked immunoadsordent assay. Result Compared with sham- operated group, Scr levels were significant increased in I/R group （ P 〈 0.05 ） , kidneys displayd significant histology changes in I/R group（ P 〈 0.05）, the amount of monocyte was significant increased in L/R group （ P 〈 0.05 ）, MDA and IL-1 were significant increased in L/R group （ P 〈 0. 05 ）, GPx was significant decreased in I/R group （ P 〈 0. 05 ）. Compared with I/R group, Set was decreased in I/R ＋ Apocynin groups, the amount of monocyte was decreased in I/R ＋ Apocynin groups（P 〈0.05） , MDA and IL-1 were decreased in I/R ＋ Apocynin groups（P 〈0. 05）. GPx was increased in I/R ＋ Apocynin groups （ P 〈 0.05 ）. Conclusions Apocynin protect kidney against ischemia/reperfusion and accelerate recovery after acute renal ischemia/reperfusion injury, which may associate with the anti-oxidation and anti-inflammation